Catalog Number - 68018-P50-2503M

The 68018‑P50‑2503M Cogent RP C18 Wide Pore Process HPLC Column is engineered for maximum‑resolution biomolecule purification in industrial and manufacturing environments, combining 10 µm particles with a 300 Å wide‑pore structure to deliver exceptional durability, extremely high loading capacity, and efficient analyte accessibility under continuous, high‑throughput conditions.

Manufactured using high‑purity 300 Å silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible performance across large‑batch and continuous process workflows

The 300 Å pore structure allows large biomolecules to fully penetrate the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å materials.

This column is ideal as a USP L1 alternate for large‑molecule separations and as a top‑tier solution for industrial biomolecule purification and process chromatography.

250 mm Length – Maximum Separation & Highest Purity

• Highest resolving power in the 50 mm wide‑pore C18 (10 µm) process range

• Maximizes peak capacity for highly complex biomolecule mixtures

• Ideal for proteins, peptides, and closely related macromolecules

• Provides extended retention for difficult and hydrophobic analytes

• Best suited for final purification and highest‑purity isolation at industrial scale

This configuration is selected when no shorter column can achieve required resolution or product purity in process workflows.

50 mm ID – Maximum Industrial‑Scale Loading

• Provides extremely high sample loading capacity (absolute top tier across entire column ecosystem)

• Designed for industrial purification, batch manufacturing, and continuous processing workflows

• Supports ultra‑high‑flow chromatography systems

Typical Flow Rate Range

• ~50–150 mL/min (system dependent)

• Optimize based on process demands and system capability

✅ Designed for process HPLC systems capable of ultra‑high flow rates and very large injection volumes

Flow Rate & System Considerations (50 mm ID, 10 µm, 300 Å)

• Requires systems capable of:

  • Ultra‑high flow rates

  • Very large injection volumes

• Compatible with:

  • Industrial chromatography systems

  • High‑capacity pumps and injection platforms

  • Large‑volume fraction collection or continuous processing systems

⚠️ Not suitable for analytical, semi‑prep, or standard preparative HPLC systems

Why Choose Wide Pore (300 Å) C18 (10 µm, Process Scale)

Designed for Biomolecules

• Enables purification of:

  • Proteins

  • Peptides

  • Large hydrophobic compounds

• Eliminates mass transfer limitations associated with smaller pore materials

Maximum‑Resolution Industrial Platform

• Ideal for final‑stage purification at manufacturing scale

• Maximizes separation efficiency, recovery, and product purity

• Supports high‑purity biomolecule production workflows

Full Workflow Scalability

• Predictable selectivity across analytical → semi‑prep → prep → production → process

• Enables efficient method transfer and scale‑up into industrial operation

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances robustness under high‑load industrial conditions

High Purity Silica

• Consistent chromatographic performance

• Reduced variability across large‑scale and continuous manufacturing runs

Durable & Cost‑Efficient

• Long column lifetime

• Designed for continuous, high‑throughput process workflows

• Reduces downtime and overall operational costs

Typical Applications

• Industrial‑scale biomolecule purification

• Protein and peptide separations

• High‑purity compound manufacturing

• Pharmaceutical production

• Process chromatography and scale‑up

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for 300 Å wide‑pore biomolecule applications

• Requires process‑scale HPLC system capability (50 mm ID)

• Ideal for maximum‑resolution, high‑purity industrial workflows

• Optimize for resolution vs throughput based on production objectives

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.