CUSTOM ITEM: HPLC Column, RP PFP, 10um, 4.0mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
68022-25P-4 Cogent RP PFP™ HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for complex analytical separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using ultra‑high purity silica.
The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it especially effective for halogenated, aromatic, and polar analytes. It is also suitable as an alternate column for USP methods requiring L43 classification.
With a 10 µm particle size, this column offers very low backpressure compared to smaller particle columns, allowing higher flow rates and improved compatibility with systems that have pressure limitations.
250 mm Length – Maximum Resolution with High Flow Capability
The 250 mm length provides the highest resolving power available in this format, ideal for complex mixtures and difficult separations.
Maximum resolution compared to shorter column lengths
Superior separation of closely related and co-eluting compounds
Highest peak capacity for complex analytical samples
✅ Recommended for impurity profiling, method development, and high-resolution workflows
Flow Rate & System Requirements (4.0 mm ID, 10 µm)
⚠️ Designed for low-pressure, high-flow operation
Typical flow rate range: ~0.8 – 1.8 mL/min
Very low backpressure vs 5 µm and 3 µm columns
Increased pressure due to extended column length, but still manageable
Compatible with standard analytical detectors
✅ Best Practice: Filter samples using a 0.2 µm syringe filter prior to injection
Why Choose PFP (F5) Columns for Selective Separations
Enhanced Retention for Halogenated Compounds
Strong interaction with halogenated and fluorinated analytes
Improved selectivity vs C18 columns
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole interactions and hydrogen bonding
Greater flexibility for method development
100 Å Pore Structure
Suitable for small to moderately sized molecules
Broad applicability across analytical workflows
Cogent RP PFP™ Column Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry
Low Backpressure (10 µm Particles)
Enables high flow rate operation
Reduces system strain and increases robustness
High Purity Silica
Consistent chromatographic performance
Reliable reproducibility
Typical Applications
High‑flow analytical methods
Halogenated compound analysis
Aromatic compound separations
Impurity profiling and method development
USP L43 methods
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Designed for standard analytical HPLC systems (4.0 mm ID)
Very low backpressure compared to smaller particle sizes
Increased pressure due to 250 mm length
Use 0.2 µm filtration prior to injection
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable