Prep HPLC Column, RP PFP, 10um, 100A. 30mm ID x 250mm Length. Cogent Brand. 1 EA.

Additional Info:

68022-P30-250 Cogent RP PFP™ Prep HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for large‑scale preparative and production workflows. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using high purity 100 Å silica.

The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it highly effective for halogenated, aromatic, and polar analytes. These mechanisms allow for improved separation of closely related compounds, which is critical in high‑purity and complex preparative separations.

With a 10 µm particle size, this column offers extremely low backpressure compared to smaller particle columns, enabling very high flow rates and improved compatibility with large‑diameter preparative HPLC systems.

250 mm Length – Maximum Resolution for Large‑Scale Purification

The 250 mm length delivers the highest resolving power available for 30 mm preparative columns, making it ideal for the most demanding purification challenges.

  • Maximum resolution vs shorter column formats

  • Superior separation of co‑eluting and structurally similar compounds

  • Highest peak capacity for complex mixtures

✅ Recommended for impurity isolation, complex separations, and high‑purity production workflows

Flow Rate & System Requirements (30 mm ID, 10 µm)

⚠️ Requires preparative or process‑scale HPLC system capability

  • Typical flow rate range: ~20 – 60 mL/min

  • Extremely low backpressure vs 5 µm and 3 µm columns

  • Increased pressure due to 250 mm length, but still manageable

  • Compatible with large‑scale fraction collection systems

✅ Important: A specialized preparative or process‑scale HPLC system is required for columns of this size

✅ Best Practice: Use proper sample filtration and a matched RP PFP guard column cartridge to protect the column and maintain long-term performance

Why Choose PFP (F5) Columns for Preparative Work

Enhanced Selectivity for Complex Mixtures

  • Strong interaction with halogenated and fluorinated compounds

  • Improved selectivity vs traditional C18 columns

Multiple Interaction Mechanisms

  • π‑π interactions for aromatic compounds

  • Dipole interactions and hydrogen bonding

  • Greater flexibility for purification method development

100 Å Pore Structure

  • Suitable for small to moderately sized molecules

  • Consistent performance from analytical to large‑scale preparative workflows

Cogent RP PFP™ Column Advantages

Fully End‑Capped Surface

  • Minimizes silanol activity

  • Improves peak symmetry and compound recovery

Ultra‑Low Backpressure (10 µm Particles)

  • Enables very high flow operation

  • Reduces system strain in large‑scale HPLC setups

High Purity Silica

  • Reliable, reproducible chromatographic performance

  • Consistent purification results at production scale

Typical Applications

  • Large‑scale preparative HPLC purification

  • Process-scale compound isolation

  • Impurity isolation and high‑purity production

  • Halogenated compound separations

  • Aromatic compound purification

Important Usage Notes

  • Packed and shipped in reversed‑phase solvents

  • Requires preparative or process‑scale HPLC system capability

  • Designed for high‑flow operation (30 mm ID columns)

  • Increased pressure due to longer column length

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

  • Columns are manufactured specifically to order

  • Orders cannot be canceled once placed

  • Custom columns are non‑returnable

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