Semi Prep HPLC Column, RP PFP, 10um, 100A. 10mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
68022-SP250 Cogent RP PFP™ Semi Prep HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for demanding purification and scale‑up applications. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using high purity 100 Å silica.
The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it highly effective for halogenated, aromatic, and polar analytes. These capabilities are essential for resolving structurally similar compounds and improving purity in preparative workflows.
With a 10 µm particle size, this column provides very low backpressure compared to smaller particle columns, allowing for higher flow rates and improved system compatibility in preparative HPLC environments.
250 mm Length – Maximum Resolution for Purification
The 250 mm length is designed for the most demanding separations, delivering the highest resolving power in semi‑prep applications.
Maximum resolution compared to shorter column lengths
Superior separation of closely related and co‑eluting compounds
Highest peak capacity for complex sample matrices
✅ Recommended for impurity isolation, complex mixtures, and high‑resolution purification workflows
Flow Rate & System Requirements (10 mm ID, 10 µm)
⚠️ Requires semi‑preparative or preparative HPLC system capability
Typical flow rate range: ~3.0 – 6.0 mL/min
Very low backpressure compared to 5 µm and 3 µm columns
Increased pressure due to 250 mm length, but still manageable
Compatible with fraction collectors and preparative detectors
✅ Important: A specialized HPLC system capable of semi‑prep or preparative flow rates is required
✅ Best Practice: Use appropriate sample filtration and/or a matched guard column to maintain performance and extend column life
Why Choose PFP (F5) Columns for Semi‑Prep Work
Enhanced Selectivity for Complex Samples
Strong interaction with halogenated and fluorinated compounds
Improved selectivity vs C18 columns
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility for purification method development
100 Å Pore Structure
Suitable for small to moderately sized molecules
Consistent selectivity from analytical to preparative scale
Cogent RP PFP™ Column Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry and compound recovery
Low Backpressure (10 µm Particles)
Enables high flow rate operation
Reduces system strain and supports scale‑up
High Purity Silica
Consistent chromatographic performance
Reliable, reproducible purification results
Typical Applications
Semi‑prep purification workflows
Impurity isolation and compound purification
Halogenated compound separations
Aromatic compound isolation
Pharmaceutical development and scale‑up
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Requires semi‑prep or preparative HPLC system
Designed for high‑flow operation (10 mm ID columns)
Increased pressure due to longer column length
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable