Catalog Number - 68034-05P-3M

Cogent RP Cyano Wide Pore is a versatile reversed-phase stationary phase designed for HPLC methods requiring lower hydrophobic retention, rapid equilibration, and unique selectivity compared to traditional C18 columns. Manufactured using high-purity silica with a cyanopropyl (CN) bonded phase, it provides balanced retention for polar and moderately hydrophobic compounds while offering compatibility with many USP L10 methods. The 300 Å wide pore structure improves accessibility for larger molecules, making it particularly useful for biomolecules and other high molecular weight analytes.

68034-05P-3M is an analytical HPLC column packed with 10 µm RP Cyano Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 50 mm format. The short column length is optimized for fast analyses, rapid method development, and scouting studies. The 10 µm particle size supports lower operating backpressure and allows higher flow rates when appropriate. Typical operating flow rates for a 4.6 mm ID column are approximately 1.0–2.0 mL/min depending on mobile phase composition, analyte characteristics, and system configuration.

This column is commonly used for proteins, peptides, enzymes, natural products, and other larger polar or neutral compounds. It is also frequently selected for USP L10 methods and preparative method scouting involving larger molecules prior to scale-up.

Key Benefits

• RP Cyano Wide Pore stationary phase

• USP L10 column equivalent chemistry

• Cyanopropyl bonded phase

• 300 Å pore size for larger molecules

• High-purity silica support

• Alternative selectivity to C18 columns

• Faster equilibration than many traditional reversed-phase columns

• Reduced hydrophobic retention

• Suitable for polar and neutral compounds

• Useful for method development and preparative scouting

Typical Applications

• USP L10 methods

• Preparative method scouting

• Peptide analysis

• Protein separations

• Enzyme analysis

• Natural product analysis

• Polar compound analysis

• Pharmaceutical analysis

• Method development

• Biomolecule separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Cyano Wide Pore

• USP Classification: L10

• Chromatography Mode: Reversed Phase

• Bonded Phase: Cyanopropyl (CN)

• Endcapping: Not Endcapped

• Particle Size: 10 µm

• Pore Size: 300 Å

• Column Dimensions: 4.6 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Additional Resources

• View Cogent Normal Phase Cyano Columns Packed in Normal Phase Solvents

Important Notes

• Packed and shipped in reversed-phase solvents

• Wide pore design improves accessibility for proteins, peptides, and other larger molecules

• Short column length is ideal for rapid analyses and method scouting

• Commonly used for preparative method development involving larger compounds

• Suitable for transferring promising methods to larger-scale columns

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