HPLC Column, RP Cyano, Wide Pore, 10um, 2.1mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Cyano Wide Pore is a versatile reversed-phase stationary phase designed for HPLC methods requiring lower hydrophobic retention, rapid equilibration, and selectivity different from traditional C18 columns. Manufactured using high-purity silica with a cyanopropyl (CN) bonded phase, it is widely used as an alternative for USP L10 methods. The 300 Å wide pore structure provides improved accessibility for larger molecules, making it particularly suitable for proteins, peptides, enzymes, and other high molecular weight analytes.
68034-05P-4-3M is an analytical HPLC column packed with 10 µm RP Cyano Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 50 mm format. The short column length is ideal for rapid analyses, fast method development, and preparative method scouting. The 10 µm particle size provides lower operating backpressure while the narrow internal diameter is well suited for low-flow applications. Typical operating flow rates are approximately 0.2–0.3 mL/min. Standard HPLC systems used with 2.1 mm ID columns must be capable of delivering reliable low flow rates and maintaining accurate solvent delivery.
This column is commonly used for proteins, peptides, enzymes, natural products, and other larger polar or neutral compounds. It is also frequently selected as a scouting column for preparative method development involving large molecules where USP L10 selectivity is desired.
Key Benefits
RP Cyano Wide Pore stationary phase
USP L10 column equivalent chemistry
Cyanopropyl bonded phase
300 Å pore size for larger molecules
High-purity silica support
Alternative selectivity to C18 columns
Faster equilibration than many traditional reversed-phase columns
Reduced hydrophobic retention
Suitable for polar and neutral compounds
Useful for preparative method development and scouting
Typical Applications
USP L10 methods
Preparative method scouting
Peptide analysis
Protein separations
Enzyme analysis
Natural product analysis
Polar compound analysis
Pharmaceutical analysis
Method development
Biomolecule separations
Specifications
Product Type: HPLC Column
Stationary Phase: RP Cyano Wide Pore
USP Classification: L10
Chromatography Mode: Reversed Phase
Bonded Phase: Cyanopropyl (CN)
Endcapping: Not Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 2.1 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 0.2–0.3 mL/min
Brand: Cogent
Additional Resources
Special Order Item Information
Important Notes
Packed and shipped in reversed-phase solvents
Wide pore design improves accessibility for proteins, peptides, enzymes, and other larger molecules
Short column length is ideal for rapid analyses and method scouting
Suitable for preparative method development involving large compounds
Requires an HPLC system capable of accurate and reproducible low-flow operation for 2.1 mm ID columns