Catalog Number - 68034-10P-3M

Cogent RP Cyano Wide Pore is a versatile reversed-phase stationary phase designed for HPLC methods requiring lower hydrophobic retention, rapid equilibration, and selectivity different from traditional C18 columns. Manufactured using high-purity silica with a cyanopropyl (CN) bonded phase, it provides balanced retention for polar and moderately hydrophobic compounds while serving as an effective alternative for USP L10 methods. The 300 Å wide pore structure improves analyte accessibility for larger molecules, making it an excellent choice for biomolecules and other high molecular weight compounds.

68034-10P-3M is an analytical HPLC column packed with 10 µm RP Cyano Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 100 mm format. The 100 mm length provides increased resolving power compared to shorter columns while maintaining efficient analysis times. The 10 µm particle size supports lower operating backpressure and allows higher flow rates when appropriate. Typical operating flow rates are approximately 1.0–2.0 mL/min depending on mobile phase composition, analyte characteristics, and instrument configuration.

This column is commonly used for proteins, peptides, enzymes, natural products, and other larger polar or neutral compounds. It is frequently selected for USP L10 methods, biomolecule analysis, pharmaceutical development, and preparative method scouting involving larger molecules.

Key Benefits

• RP Cyano Wide Pore stationary phase

• USP L10 column equivalent chemistry

• Cyanopropyl bonded phase

• 300 Å pore size for larger molecules

• High-purity silica support

• Alternative selectivity to C18 columns

• Faster equilibration than many traditional reversed-phase columns

• Reduced hydrophobic retention

• Suitable for polar and neutral compounds

• Excellent for biomolecule separations and method development

Typical Applications

• USP L10 methods

• Peptide analysis

• Protein separations

• Enzyme analysis

• Natural product analysis

• Pharmaceutical analysis

• Biomolecule characterization

• Method development

• Preparative method scouting

• Quality control testing

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Cyano Wide Pore

• USP Classification: L10

• Chromatography Mode: Reversed Phase

• Bonded Phase: Cyanopropyl (CN)

• Endcapping: Not Endcapped

• Particle Size: 10 µm

• Pore Size: 300 Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Additional Resources

• View Cogent Normal Phase Cyano Columns Packed in Normal Phase Solvents

Important Notes

• Packed and shipped in reversed-phase solvents

• Wide pore design improves accessibility for proteins, peptides, enzymes, and other larger molecules

• Increased column length provides improved resolution compared to shorter formats

• Frequently selected for USP L10 methods involving larger analytes

• Useful for preparative method scouting and method transfer studies

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