Catalog Number - 68034-25P

Cogent RP Cyano is a versatile reversed-phase stationary phase designed for HPLC methods requiring lower hydrophobic retention, rapid equilibration, and selectivity different from traditional C18 columns. Manufactured using high-purity silica with a cyanopropyl (CN) bonded phase, it provides balanced retention for polar and moderately hydrophobic compounds while serving as an effective alternative for USP L10 methods. The unique cyano functionality often delivers different separation characteristics than alkyl-bonded phases, making it valuable for method development and selectivity screening.

68034-25P is an analytical HPLC column packed with 10 µm RP Cyano particles featuring a 100 Å pore size in a 4.6 mm ID x 250 mm format. The 250 mm column length maximizes resolving power and peak capacity for complex separations where enhanced resolution is required. The 10 µm particle size supports lower operating backpressure and allows higher flow rates when appropriate. Typical operating flow rates are approximately 1.0–2.0 mL/min depending on mobile phase composition, analyte characteristics, and instrument configuration.

This column is commonly used for polar and neutral compounds where reduced retention and faster equilibration are desirable. It is frequently selected for USP L10 methods, pharmaceutical analysis, impurity profiling, quality control testing, and method development projects requiring maximum chromatographic resolution and alternative reversed-phase selectivity.

Key Benefits

• RP Cyano stationary phase

• USP L10 column equivalent chemistry

• Cyanopropyl bonded phase

• High-purity silica support

• Alternative selectivity to C18 columns

• Faster equilibration than many traditional reversed-phase columns

• Reduced hydrophobic retention

• Suitable for polar and neutral compounds

• Lower operating backpressure with 10 µm particles

• Maximum resolving power for complex separations

Typical Applications

• USP L10 methods

• Pharmaceutical analysis

• Impurity profiling

• Method development

• Quality control testing

• Polar compound analysis

• Neutral compound analysis

• Alternative selectivity screening

• Routine HPLC analysis

• Preparative method scouting

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Cyano

• USP Classification: L10

• Chromatography Mode: Reversed Phase

• Bonded Phase: Cyanopropyl (CN)

• Endcapping: Not Endcapped

• Particle Size: 10 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Typical Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Additional Resources

• View Cogent Normal Phase Cyano Columns Packed in Normal Phase Solvents

Important Notes

• Packed and shipped in reversed-phase solvents

• Commonly used as an alternative selectivity choice to traditional C18 columns

• Extended column length maximizes resolution and peak capacity

• Frequently selected for USP L10 methods

• Useful for preparative method scouting and method transfer studies

  ---