HPLC Column, RP Cyano, Wide Pore, 10um, 4.0mm ID x 250mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Cyano Wide Pore is a versatile reversed-phase stationary phase designed for HPLC methods requiring lower hydrophobic retention, rapid equilibration, and alternative selectivity compared to traditional C18 columns. Manufactured using high-purity silica with a cyanopropyl (CN) bonded phase, it provides balanced retention for polar and moderately hydrophobic compounds while serving as an effective USP L10 alternative. The 300 Å wide pore structure improves analyte accessibility for larger molecules, making it particularly useful for proteins, peptides, enzymes, and other high molecular weight compounds.
68034-25P-4-3M is an analytical HPLC column packed with 10 µm RP Cyano Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 250 mm format. The 250 mm column length maximizes resolving power and peak capacity for complex separations where the highest chromatographic resolution is required. The 10 µm particle size supports lower operating backpressure and allows higher flow rates when appropriate. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, analyte characteristics, and instrument configuration.
This column is commonly used for proteins, peptides, enzymes, natural products, and other larger polar or neutral compounds. It is frequently selected for USP L10 methods, biomolecule analysis, pharmaceutical development, impurity profiling, and preparative method scouting involving larger molecules where maximum chromatographic performance is desired.
Key Benefits
RP Cyano Wide Pore stationary phase
USP L10 column equivalent chemistry
Cyanopropyl bonded phase
300 Å pore size for larger molecules
High-purity silica support
Alternative selectivity to C18 columns
Faster equilibration than many traditional reversed-phase columns
Reduced hydrophobic retention
Suitable for polar and neutral compounds
Maximum resolving power for biomolecule separations
Typical Applications
USP L10 methods
Peptide analysis
Protein separations
Enzyme analysis
Natural product analysis
Pharmaceutical analysis
Biomolecule characterization
Impurity profiling
Method development
Preparative method scouting
Specifications
Product Type: HPLC Column
Stationary Phase: RP Cyano Wide Pore
USP Classification: L10
Chromatography Mode: Reversed Phase
Bonded Phase: Cyanopropyl (CN)
Endcapping: Not Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Additional Resources
Special Order Item Information
Important Notes
Packed and shipped in reversed-phase solvents
Wide pore design improves accessibility for proteins, peptides, enzymes, and other larger molecules
Extended column length maximizes resolution and peak capacity
Frequently selected for USP L10 methods involving larger analytes
Useful for preparative method scouting and method transfer studies
This is a custom manufactured item and cannot be canceled or returned once ordered