Catalog Number - 68039-25P

Cogent RP Phenyl Hexyl is a highly selective reversed-phase stationary phase designed for enhanced aromatic compound separations. Manufactured on high-purity silica with a fully double endcapped phenyl hexyl (C6-Phenyl) bonded phase, it combines hydrophobic retention with strong π-π interactions. The extended six-carbon linker positions the phenyl ring farther from the silica surface, reducing silanol influence and often providing different selectivity than conventional phenyl phases. This chemistry is commonly used as an alternative for certain USP L11 methods and for challenging aromatic compound separations.

68039-25P is an analytical HPLC column packed with 10 µm RP Phenyl Hexyl particles featuring a 100 Å pore size in a 4.6 mm ID x 250 mm format. The 250 mm column length maximizes resolving power and peak capacity for complex separations where the highest chromatographic resolution is required. The 10 µm particle size supports higher flow rates with lower operating backpressure, making this column suitable for impurity profiling, method development, difficult separations, and preparative method scouting. Typical operating flow rates are approximately 1.0–2.0 mL/min depending on mobile phase composition, analyte characteristics, and system configuration.

This column is particularly effective for aromatic compounds, positional isomers, tocopherols, flavonoids, polynuclear aromatic compounds, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds containing aromatic rings. For enhanced π-π interactions and selectivity, methanol is often preferred over acetonitrile as the organic modifier. Performance is comparable to Restek® Phenyl Hexyl HPLC columns of similar dimensions and phase chemistry.

Key Benefits

• RP Phenyl Hexyl stationary phase

• USP L11 alternative chemistry

• C6-Phenyl bonded phase

• Fully double endcapped

• High-purity silica support

• Enhanced π-π interaction selectivity

• Reduced silanol interaction effects

• Alternative selectivity to conventional phenyl columns

• Lower operating backpressure with 10 µm particles

• Comparable performance to Restek® Phenyl Hexyl columns

Typical Applications

• USP L11 methods

• Aromatic compound analysis

• Positional isomer separations

• Active pharmaceutical ingredient (API) analysis

• Flavonoid analysis

• Tocopherol analysis

• Polynuclear aromatic compound separations

• Nitro-aromatic compound analysis

• Natural product analysis

• Preparative method scouting

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Phenyl Hexyl

• USP Classification: L11 Alternative

• Chromatography Mode: Reversed Phase

• Bonded Phase: Phenyl Hexyl (C6-Phenyl)

• Endcapping: Fully Double Endcapped

• Particle Size: 10 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Typical Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Additional Resources

• View Specifications of Cogent Reversed Phase Columns

Important Notes

• Often selected as an alternative for USP L11 methods

• Extended C6 linker can provide different selectivity than conventional phenyl phases

• Methanol is often preferred over acetonitrile to maximize π-π interactions

• Extended column length maximizes resolution and peak capacity for complex separations

• Performance is comparable to Restek® Phenyl Hexyl HPLC columns of similar dimensions and phase chemistry

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