Catalog Number - 68134-05P

Cogent RP Cyano™ is a versatile reversed-phase UHPLC stationary phase designed for polar and neutral compound separations where reduced retention and rapid equilibration are desirable. Manufactured with a cyanopropyl bonded phase on ultra-high purity silica and supplied without endcapping, this chemistry provides unique selectivity compared to traditional alkyl phases. RP Cyano™ is commonly used as an alternative for USP L10 methods and offers shorter retention times than many conventional reversed-phase columns while maintaining excellent chromatographic performance.

68134-05P is a UHPLC column packed with 1.8 µm RP Cyano™ particles featuring a 100 Å pore size in a 4.6 mm ID x 50 mm format. The short 50 mm length is optimized for rapid analyses and high sample throughput, while the sub-2 µm particle technology delivers exceptional efficiency and peak capacity. This column is specifically engineered for high-pressure UHPLC applications and is designed to take full advantage of UHPLC instrumentation capable of operating at the elevated pressures generated by 1.8 µm particles. Typical operating flow rates are approximately 1.0–2.0 mL/min depending on method conditions and instrument pressure limitations.

This column is particularly useful for polar and moderately hydrophobic compounds requiring reversed-phase retention with faster equilibration and shorter run times. Packed and shipped in reversed-phase solvents, it is intended for reversed-phase chromatography applications.

Key Benefits

• RP Cyano™ stationary phase

• USP L10 equivalent chemistry

• Cyanopropyl bonded phase

• Ultra-high purity silica support

• Designed for UHPLC applications

• High-efficiency 1.8 µm particles

• Unique selectivity versus C18 and C8 phases

• Shorter retention times for many analytes

• Rapid equilibration between runs

• Excellent efficiency and peak capacity

Typical Applications

• USP L10 methods

• UHPLC analysis

• Polar compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

• Method development

• Impurity profiling

• High-throughput laboratories

• Fast reversed-phase separations

• Quality control testing

Specifications

• Product Type: UHPLC Column

• Stationary Phase: RP Cyano™

• USP Classification: L10

• Chromatography Mode: Reversed Phase

• Bonded Phase: Cyanopropyl

• Endcapping: Not Endcapped

• Particle Size: 1.8 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed specifically for UHPLC operation and high-pressure reversed-phase chromatography

• Delivers maximum performance when used on a UHPLC system designed for sub-2 µm particle columns

• While compatible HPLC systems may be used in some applications, the column is engineered primarily for UHPLC operation

• Sub-2 µm particles generate significantly higher backpressure than conventional HPLC columns

• Packed and shipped in reversed-phase solvents

• For Normal Phase Cyano columns packed in non-polar solvents, see the Cogent NP Cyano product line

• Samples should be filtered through a 0.2 µm syringe filter prior to injection

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