UHPLC Column, RP Cyano, 1.8um, 100A. 2.1mm ID x 50mm Length. Cogent Brand. 1 EA.

Additional Information:

Cogent RP Cyano™ is a versatile reversed-phase UHPLC stationary phase designed for polar and neutral compound separations where reduced retention and rapid equilibration are desired. Manufactured with a cyanopropyl bonded phase on ultra-high purity silica and supplied without endcapping, this chemistry provides distinct selectivity compared to traditional alkyl phases. RP Cyano™ is commonly used as an alternative for USP L10 methods and offers shorter retention times than many conventional reversed-phase columns while maintaining excellent chromatographic performance.

68134-05P-2 is a UHPLC column packed with 1.8 µm RP Cyano™ particles featuring a 100 Å pore size in a 2.1 mm ID x 50 mm format. The short 50 mm length is optimized for rapid analyses and high sample throughput, while the sub-2 µm particle technology provides exceptional efficiency and increased peak capacity. This column is specifically engineered for high-pressure UHPLC applications and is designed to take full advantage of UHPLC instrumentation capable of operating at the elevated pressures generated by 1.8 µm particles. Typical operating flow rates are approximately 0.2–0.4 mL/min. Because of the low flow rates required by 2.1 mm ID columns, standard HPLC systems must be capable of precise and reproducible low-flow operation if used with this column.

This column is particularly useful for polar and neutral compounds requiring reversed-phase retention with fast equilibration and efficient separations. The 2.1 mm ID format is commonly used for LC-MS applications due to reduced solvent consumption and excellent compatibility with mass spectrometric detection.

Key Benefits

  • RP Cyano™ stationary phase

  • USP L10 equivalent chemistry

  • Cyanopropyl bonded phase

  • Ultra-high purity silica support

  • Designed for UHPLC applications

  • High-efficiency 1.8 µm particles

  • Unique selectivity compared to C8 and C18 phases

  • Shorter retention times for many analytes

  • Rapid equilibration between runs

  • Ideal for LC-MS methods

Typical Applications

  • USP L10 methods

  • UHPLC analysis

  • LC-MS methods

  • Polar compound analysis

  • Neutral compound analysis

  • Pharmaceutical analysis

  • Method development

  • Impurity profiling

  • Fast reversed-phase separations

  • Quality control testing

Specifications

  • Product Type: UHPLC Column

  • Stationary Phase: RP Cyano™

  • USP Classification: L10

  • Chromatography Mode: Reversed Phase

  • Bonded Phase: Cyanopropyl

  • Endcapping: Not Endcapped

  • Particle Size: 1.8 µm

  • Pore Size: 100 Å

  • Column Dimensions: 2.1 mm ID x 50 mm

  • Length: 50 mm

  • Typical Flow Rate: 0.2–0.4 mL/min

  • Brand: Cogent

Important Notes

  • Designed specifically for UHPLC operation and high-pressure reversed-phase chromatography

  • 1.8 µm particles generate substantially higher backpressure than conventional HPLC columns

  • Maximum performance is achieved on UHPLC systems designed for sub-2 µm particle technology

  • The 2.1 mm ID format is commonly used for LC-MS applications

  • Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.4 mL/min

  • Packed and shipped in reversed-phase solvents

  • For Normal Phase Cyano columns packed in non-polar solvents, see the Cogent NP Cyano product line

  • Samples should be filtered through a 0.2 µm syringe filter prior to injection


 

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