Catalog Number - 68134-15P-2

Cogent RP Cyano™ is a versatile reversed-phase UHPLC stationary phase designed for polar and neutral compound separations where reduced retention and rapid equilibration are desired. Manufactured with a cyanopropyl bonded phase on ultra-high purity silica and supplied without endcapping, this chemistry provides distinct selectivity compared to traditional alkyl phases. RP Cyano™ is commonly used as an alternative for USP L10 methods and offers shorter retention times than many conventional reversed-phase columns while maintaining excellent chromatographic performance.

68134-15P-2 is a UHPLC column packed with 1.8 µm RP Cyano™ particles featuring a 100 Å pore size in a 2.1 mm ID x 150 mm format. The 150 mm length maximizes resolution and peak capacity for complex sample separations, while the sub-2 µm particle technology delivers exceptional efficiency. This column is specifically engineered for high-pressure UHPLC operation and is designed to take full advantage of UHPLC instrumentation capable of operating at the elevated pressures generated by 1.8 µm particles. Typical operating flow rates are approximately 0.2–0.4 mL/min. Because of the low flow rates required by 2.1 mm ID columns, standard HPLC systems must be capable of precise and reproducible low-flow operation if they are used with this column.

This column is particularly useful for polar and neutral compounds requiring reversed-phase retention with shorter retention times than many traditional alkyl phases. The combination of RP Cyano™ selectivity and UHPLC efficiency makes it an excellent choice for LC-MS methods, impurity profiling, method development, and complex sample analyses requiring maximum resolving power.

Key Benefits

• RP Cyano™ stationary phase

• USP L10 equivalent chemistry

• Cyanopropyl bonded phase

• Ultra-high purity silica support

• Designed for UHPLC applications

• High-efficiency 1.8 µm particles

• Unique selectivity compared to C8 and C18 phases

• Shorter retention times for many analytes

• Rapid equilibration between runs

• Ideal for LC-MS methods

Typical Applications

• USP L10 methods

• UHPLC analysis

• LC-MS methods

• Polar compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

• Method development

• Impurity profiling

• Complex sample separations

• Quality control testing

Specifications

• Product Type: UHPLC Column

• Stationary Phase: RP Cyano™

• USP Classification: L10

• Chromatography Mode: Reversed Phase

• Bonded Phase: Cyanopropyl

• Endcapping: Not Endcapped

• Particle Size: 1.8 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Length: 150 mm

• Typical Flow Rate: 0.2–0.4 mL/min

• Brand: Cogent

Important Notes

• Designed specifically for UHPLC operation and high-pressure reversed-phase chromatography

• 1.8 µm particles generate substantially higher backpressure than conventional HPLC columns

• Maximum performance is achieved on UHPLC systems designed for sub-2 µm particle technology

• The 2.1 mm ID format is commonly used for LC-MS applications

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.4 mL/min

• Packed and shipped in reversed-phase solvents

• View Cogent NP Cyano Columns packed in Normal Phase solvents for Normal Phase applications

• The 150 mm length maximizes resolution and peak capacity for complex sample separations

• Samples should be filtered through a 0.2 µm syringe filter prior to injection

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