Catalog Number - 68140-15P-2

Cogent NP Amino™ is a versatile normal phase and HILIC stationary phase designed for the separation of highly polar compounds that are often difficult to retain using traditional reversed-phase methods. Manufactured on ultra-high purity silica with a propyl amino bonded phase and supplied without endcapping, this chemistry provides excellent selectivity for carbohydrates, sugars, organic acids, and other polar analytes. The stationary phase is commonly used as an alternative for USP L8 methods and is suitable for both Normal Phase and HILIC applications.

68140-15P-2 is a UHPLC column packed with 1.8 µm NP Amino™ particles featuring a 100 Å pore size in a 2.1 mm ID x 150 mm format. The 150 mm length maximizes resolution and peak capacity for complex separations, while the narrow-bore design reduces solvent consumption and is frequently selected for LC-MS applications. The sub-2 µm particle technology delivers exceptional chromatographic efficiency and is specifically designed for high-pressure UHPLC operation. Typical operating flow rates are approximately 0.2–0.4 mL/min. Because of the low flow rates required by 2.1 mm ID columns, any HPLC system used with this column must be capable of precise and reproducible low-flow operation.

This column is particularly effective for sugars, carbohydrates, negatively charged compounds, organic acids, and compounds containing carboxylic acid functionality. Suitable for both Normal Phase and HILIC applications, it is an excellent choice when maximum resolution and enhanced retention of polar compounds are required.

Key Benefits

• NP Amino™ stationary phase

• USP L8 equivalent chemistry

• Propyl amino bonded phase

• Ultra-high purity silica support

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• Designed for UHPLC applications

• High-efficiency 1.8 µm particles

• Exceptional peak capacity

• Reduced solvent consumption

Typical Applications

• UHPLC analysis

• USP L8 methods

• HILIC separations

• Normal Phase chromatography

• Carbohydrate analysis

• Sugar analysis

• Organic acid analysis

• Polar compound analysis

• LC-MS methods

• Complex sample separations

Specifications

• Product Type: UHPLC Column

• Stationary Phase: NP Amino™

• USP Classification: L8

• Chromatography Mode: Normal Phase / HILIC

• Bonded Phase: Propyl Amino

• Endcapping: Not Endcapped

• Particle Size: 1.8 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Length: 150 mm

• Typical Flow Rate: 0.2–0.4 mL/min

• Brand: Cogent

Important Notes

• Specifically designed for UHPLC operation and high-pressure chromatography

• Delivers maximum performance when used on a UHPLC system designed for sub-2 µm particle columns

• May be used on compatible HPLC systems only if instrument pressure limits are not exceeded and low-flow operation is reliable

• Sub-2 µm particles generate substantially higher backpressure than conventional HPLC columns

• The 2.1 mm ID format is commonly used for LC-MS applications

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.4 mL/min

• Packed and shipped in Normal Phase solvents

• Suitable for both Normal Phase and HILIC chromatographic modes

• Excellent for sugars, carbohydrates, organic acids, and other highly polar compounds

• The 150 mm length maximizes resolution and peak capacity for complex sample separations

• Samples should be filtered through a 0.2 µm syringe filter prior to injection

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