Catalog Number - 68308-05P-2

Cogent RP C8 Octyl™ is a versatile reversed-phase HPLC stationary phase designed for the separation of hydrophobic and neutral compounds. Manufactured using a traditional octyl (C8) bonded phase on ultra-high purity silica and fully double end capped, it provides strong hydrophobic interactions while often offering reduced retention compared to C18 phases. As a USP L7 equivalent phase, it is widely used for method replacement, method development, and routine analytical separations where balanced retention and selectivity are desired.

68308-05P-2 is an HPLC column packed with 3 µm RP C8 Octyl™ particles featuring a 100 Å pore size in a 2.1 mm ID x 50 mm format. This popular narrow-bore configuration is designed for rapid separations with reduced solvent consumption. The 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns, while the short 50 mm length minimizes analysis times. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, HPLC systems must be capable of precise and reproducible low-flow operation, and detector flow cells should be appropriately configured for low-volume applications.

This column is particularly useful for hydrophobic and neutral compounds in reversed-phase chromatography and is especially well suited for long-chain fatty acid analysis. The narrow-bore format is also highly popular for LC-MS applications where lower solvent consumption, improved sensitivity, and short run times are desirable. As with all 3 µm columns, backpressure will be higher than comparable 5 µm columns, and longer columns used with viscous mobile phases may approach system pressure limitations.

Key Benefits

• RP C8 Octyl™ stationary phase

• USP L7 equivalent chemistry

• Fully double end capped

• Traditional octyl (C8) bonded phase

• Ultra-high purity silica support

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Sharp peak shapes

• Fast analysis times

• Excellent for LC-MS applications

Typical Applications

• USP L7 methods

• Reversed-phase HPLC

• LC-MS methods

• Long-chain fatty acid analysis

• Hydrophobic compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

• Method development

• Quality control testing

• Preparative method scouting

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8 Octyl™

• USP Classification: L7

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Suitable for conventional HPLC and LC-MS systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 2.1 mm ID format is commonly used for LC-MS applications

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min

• Detector flow cells should be optimized for low-volume, narrow-bore chromatography

• The 50 mm length provides rapid analysis and increased sample throughput

• Particularly useful for long-chain fatty acid separations

• Methods using longer columns and viscous mobile phases may approach instrument pressure limits

• Commonly used as an alternative for USP L7 methods

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