HPLC Column, RP C8 Octyl, Wide Pore, 3um, 4.6mm ID x 250mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C8 Octyl™ Wide Pore is a reversed-phase HPLC stationary phase specifically designed for the separation of large hydrophobic and neutral molecules. Manufactured using a traditional octyl (C8) bonded phase on ultra-high purity silica and fully double end capped, this 300 Å wide pore chemistry provides enhanced accessibility for larger analytes compared to standard 100 Å pore columns. As a USP L7 equivalent phase, it is particularly useful for proteins, peptides, long-chain fatty acids, and other compounds that benefit from improved pore accessibility and mass transfer.
68308-25P-3M is an HPLC column packed with 3 µm RP C8 Octyl™ Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 250 mm format. The 250 mm length provides maximum chromatographic resolution and peak capacity within the RP C8 Wide Pore product family, while the 3 µm particles deliver greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.
This column is particularly useful for large hydrophobic or neutral compounds analyzed by reversed-phase HPLC. The C8 chemistry offers balanced hydrophobic retention while the 300 Å pore structure improves performance for larger analytes including proteins, peptides, and long-chain fatty acids. The extended 250 mm length provides exceptional resolving power for complex biomolecule separations, biopharmaceutical analyses, and highly demanding analytical applications.
Key Benefits
RP C8 Octyl™ Wide Pore stationary phase
USP L7 equivalent chemistry
Fully double end capped
Traditional octyl (C8) bonded phase
Ultra-high purity silica support
300 Å wide pore design
Optimized for large molecules
3 µm particle technology
Maximum peak capacity
Exceptional chromatographic resolution
Typical Applications
USP L7 methods
Protein analysis
Peptide analysis
Long-chain fatty acid analysis
Large molecule separations
Biopharmaceutical analysis
Hydrophobic compound analysis
Pharmaceutical analysis
Method development
Complex sample characterization
Specifications
Product Type: HPLC Column
Stationary Phase: RP C8 Octyl™ Wide Pore
USP Classification: L7
Chromatography Mode: Reversed Phase
Bonded Phase: Octyl (C8)
Endcapping: Fully Double End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 4.6 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Important Notes
Designed for large hydrophobic and neutral molecules
300 Å wide pores improve access to the bonded phase for larger analytes
Suitable for conventional HPLC systems
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
The 250 mm length provides maximum resolution and peak capacity for demanding separations
Particularly useful for proteins, peptides, and long-chain fatty acids
Provides balanced hydrophobic retention for large molecule separations
Commonly used as an alternative for USP L7 methods involving larger analytes
Ideal for complex biomolecule characterization and biopharmaceutical analyses