HPLC Column, RP Phenyl, Wide Pore, 3um, 4.6mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Phenyl™ Wide Pore is a reversed-phase HPLC stationary phase designed to provide unique selectivity through π-π interactions between aromatic analytes and the bonded phenyl functionality while offering enhanced accessibility for larger molecules. Manufactured using a traditional three-carbon linker with a terminal phenyl ring bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore phase provides chromatographic behavior distinct from conventional alkyl phases. As a USP L11 equivalent phase, it is particularly useful for large molecules, aromatic compounds, natural products, and method development applications.
68324-05P-3M is an HPLC column packed with 3 µm RP Phenyl™ Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 50 mm format. The short 50 mm length is optimized for rapid separations and increased sample throughput, while the 3 µm particles provide improved efficiency and sharper peaks than comparable 5 µm columns. The wide pore structure improves analyte access to the bonded phase for larger compounds while maintaining the distinctive selectivity associated with phenyl chemistry. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.
This column is particularly useful for the separation of large positional isomers, natural products, related compounds, and certain peptides in reversed-phase HPLC. The unique selectivity of the phenyl phase can provide improved resolution for aromatic analytes where conventional C8 or C18 phases may not deliver adequate selectivity. The combination of wide pores, rapid analysis capability, and enhanced aromatic selectivity makes this column an excellent choice for research, pharmaceutical, and bioanalytical applications.
Key Benefits
RP Phenyl™ Wide Pore stationary phase
USP L11 equivalent chemistry
Fully double end capped
Phenyl bonded phase
Ultra-high purity silica support
300 Å wide pore design
Enhanced aromatic selectivity
Optimized for larger molecules
3 µm particle size
Fast analysis times
Typical Applications
USP L11 methods
Large positional isomer separations
Natural product analysis
Peptide analysis
Aromatic compound analysis
Method development
Bioanalytical methods
Pharmaceutical analysis
Reversed-phase HPLC
Research and development
Specifications
Product Type: HPLC Column
Stationary Phase: RP Phenyl™ Wide Pore
USP Classification: L11
Chromatography Mode: Reversed Phase
Bonded Phase: Phenyl
Endcapping: Fully Double End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 4.6 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Important Notes
Designed for larger molecules and aromatic compounds
300 Å wide pores improve access to the bonded phase for larger analytes
Suitable for conventional HPLC systems
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Generates higher backpressure than larger particle columns
The 50 mm length is ideal for rapid analyses and high-throughput workflows
Particularly useful for natural products and peptide separations
Provides unique selectivity compared to C8 and C18 phases
Commonly used as an alternative for USP L11 methods involving larger analytes