Catalog Number - 68324-10P

Cogent RP Phenyl™ is a reversed-phase HPLC stationary phase designed to provide unique selectivity through π-π interactions between aromatic analytes and the bonded phenyl functionality. Manufactured using a traditional three-carbon linker with a terminal phenyl ring bonded to ultra-high purity silica and fully double end capped, this stationary phase provides chromatographic behavior distinct from conventional alkyl phases. As a USP L11 equivalent phase, it is widely used for method replacement, method development, and applications involving aromatic and structurally related compounds.

68324-10P is an HPLC column packed with 3 µm RP Phenyl™ particles featuring a 100 Å pore size in a 4.6 mm ID x 100 mm format. The 100 mm length provides greater chromatographic resolution than shorter 50 mm columns while maintaining practical run times for routine analysis. The 3 µm particles provide improved efficiency and sharper peaks than comparable 5 µm columns, making this format an excellent balance between speed and resolving power. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.

This column is particularly useful for the separation of positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. The unique selectivity of the phenyl phase makes it an excellent choice when conventional C18 or C8 columns do not provide adequate resolution. This size is frequently selected when additional resolution is needed beyond what a 50 mm column can provide while maintaining relatively short analysis times.

Key Benefits

• RP Phenyl™ stationary phase

• USP L11 equivalent chemistry

• Fully double end capped

• Phenyl bonded phase

• Ultra-high purity silica support

• Enhanced aromatic selectivity

• Excellent isomer separation capability

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Improved chromatographic resolution

Typical Applications

• USP L11 methods

• Positional isomer separations

• Tocopherol analysis

• Flavonoid analysis

• Polynuclear aromatic hydrocarbon analysis

• Nitro-aromatic compound analysis

• Active pharmaceutical ingredient (API) analysis

• Natural product analysis

• Method development

• Reversed-phase HPLC

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Phenyl™

• USP Classification: L11

• Chromatography Mode: Reversed Phase

• Bonded Phase: Phenyl

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• Suitable for conventional HPLC systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 100 mm length provides greater resolution than 50 mm columns while maintaining relatively short run times

• Provides unique selectivity compared to C8 and C18 phases

• Particularly useful for aromatic compounds and positional isomers

• Commonly used as an alternative for USP L11 methods

• Frequently selected for method development and chromatographic optimization