HPLC Column, RP Cyano, Wide Pore, 3um, 2.1mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Cyano™ Wide Pore is a versatile reversed-phase HPLC stationary phase designed for the separation of larger polar and neutral compounds. Manufactured using a three-carbon ligand with a terminal cyano (CN) functional group bonded to ultra-high purity silica, this 300 Å wide pore stationary phase provides selectivity distinct from traditional C18, C8, and phenyl phases while offering increased accessibility for large analytes. As a USP L10 equivalent phase, it is particularly useful for proteins, peptides, enzymes, natural products, and other larger molecules that benefit from wide pore chromatography.
68334-05P-2-3M is an HPLC column packed with 3 µm RP Cyano™ Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 50 mm format. This short, narrow-bore configuration is optimized for rapid separations, reduced solvent consumption, and excellent compatibility with LC-MS instrumentation. The 300 Å pore structure improves analyte access to the bonded phase for larger compounds, while the 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, standard HPLC systems must be capable of precise and reproducible low-flow operation.
This column is particularly useful for large polar or neutral compounds including proteins, peptides, enzymes, and natural products analyzed by reversed-phase HPLC or LC-MS. Compared to non-modified silica columns, the RP Cyano™ Wide Pore phase typically provides shorter retention times and faster equilibration between runs, making it an excellent choice for high-throughput analytical and bioanalytical methods.
Key Benefits
RP Cyano™ Wide Pore stationary phase
USP L10 equivalent chemistry
Cyano bonded phase
Ultra-high purity silica support
300 Å wide pore design
Optimized for large molecules
Alternative selectivity to C18 and C8 phases
Faster equilibration between runs
3 µm particle size
Excellent for LC-MS applications
Typical Applications
USP L10 methods
LC-MS methods
Protein analysis
Peptide analysis
Enzyme analysis
Natural product analysis
Polar compound analysis
Neutral compound analysis
Bioanalytical methods
Reversed-phase HPLC
Specifications
Product Type: HPLC Column
Stationary Phase: RP Cyano™ Wide Pore
USP Classification: L10
Chromatography Mode: Reversed Phase
Bonded Phase: Cyano (CN)
Endcapping: Not End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 2.1 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 0.2–0.3 mL/min
Brand: Cogent
Important Notes
Designed for larger molecules including proteins, peptides, enzymes, and natural products
300 Å wide pores improve access to the bonded phase for large analytes
Provides alternative selectivity compared to C18, C8, and Phenyl phases
Suitable for reversed-phase HPLC and LC-MS applications
The 2.1 mm ID format is commonly used for LC-MS applications
Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min
Detector flow cells should be optimized for low-volume, narrow-bore chromatography
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Packed and shipped in reversed-phase solvents
For normal-phase applications, see the related Cogent NP Cyano Columns packed in normal-phase solvents