HPLC Column, RP Cyano, Wide Pore, 3um, 2.1mm ID x 100mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Cyano™ Wide Pore is a versatile reversed-phase HPLC stationary phase designed for the separation of larger polar and neutral compounds. Manufactured using a three-carbon ligand with a terminal cyano (CN) functional group bonded to ultra-high purity silica, this 300 Å wide pore stationary phase provides selectivity distinct from traditional C18, C8, and phenyl phases while offering increased accessibility for large analytes. As a USP L10 equivalent phase, it is particularly useful for proteins, peptides, enzymes, natural products, and other larger molecules that benefit from wide pore chromatography.
68334-10P-2-3M is an HPLC column packed with 3 µm RP Cyano™ Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 100 mm format. This narrow-bore configuration combines enhanced chromatographic resolution with reduced solvent consumption and excellent compatibility with LC-MS instrumentation. The 100 mm length provides greater resolving power than shorter 50 mm columns, while the wide pore structure improves analyte access to the bonded phase for larger compounds. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, standard HPLC systems must be capable of precise and reproducible low-flow operation.
This column is particularly useful for large polar or neutral compounds including proteins, peptides, enzymes, and natural products analyzed by reversed-phase HPLC and LC-MS methods. Compared to non-modified silica columns, the RP Cyano™ Wide Pore phase typically provides shorter retention times and faster equilibration between runs, making it an excellent choice for bioanalytical, pharmaceutical, and research applications requiring enhanced resolution and alternative selectivity.
Key Benefits
RP Cyano™ Wide Pore stationary phase
USP L10 equivalent chemistry
Cyano bonded phase
Ultra-high purity silica support
300 Å wide pore design
Optimized for large molecules
Alternative selectivity to C18 and C8 phases
Faster equilibration between runs
3 µm particle size
Excellent for LC-MS applications
Typical Applications
USP L10 methods
LC-MS methods
Protein analysis
Peptide analysis
Enzyme analysis
Natural product analysis
Polar compound analysis
Neutral compound analysis
Bioanalytical methods
Reversed-phase HPLC
Specifications
Product Type: HPLC Column
Stationary Phase: RP Cyano™ Wide Pore
USP Classification: L10
Chromatography Mode: Reversed Phase
Bonded Phase: Cyano (CN)
Endcapping: Not End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 2.1 mm ID x 100 mm
Length: 100 mm
Typical Flow Rate: 0.2–0.3 mL/min
Brand: Cogent
Important Notes
Designed for larger molecules including proteins, peptides, enzymes, and natural products
300 Å wide pores improve access to the bonded phase for large analytes
Provides alternative selectivity compared to C18, C8, and Phenyl phases
Suitable for reversed-phase HPLC and LC-MS applications
The 2.1 mm ID format is commonly used for LC-MS applications
Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min
Detector flow cells should be optimized for low-volume, narrow-bore chromatography
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
The 100 mm length provides greater resolution than 50 mm columns while maintaining practical run times
Packed and shipped in reversed-phase solvents
For normal-phase applications, see the related Cogent NP Cyano Columns packed in normal-phase solvents