Catalog Number - 68334-10P-4

Cogent RP Cyano™ is a versatile reversed-phase HPLC stationary phase designed for the separation of polar and neutral compounds. Manufactured using a three-carbon ligand with a terminal cyano (CN) functional group bonded to ultra-high purity silica, this stationary phase provides selectivity distinct from traditional C18, C8, and phenyl phases. As a USP L10 equivalent phase, it is widely used for method replacement, method development, and applications requiring reduced hydrophobic retention, faster equilibration, and alternative selectivity.

68334-10P-4 is an HPLC column packed with 3 µm RP Cyano™ particles featuring a 100 Å pore size in a 4.0 mm ID x 100 mm format. The 100 mm length provides greater chromatographic resolution than shorter 50 mm columns while maintaining practical analysis times for routine laboratory use. The 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.

Often selected for polar and neutral compounds in reversed-phase HPLC, this Cyano phase typically provides shorter retention times and faster equilibration than non-modified silica columns. The 100 mm format offers an excellent balance between speed and resolution, making it suitable for pharmaceutical analysis, method development, and routine quality control applications.

Key Benefits

• RP Cyano™ stationary phase

• USP L10 equivalent chemistry

• Cyano bonded phase

• Ultra-high purity silica support

• Alternative selectivity to C18 and C8 phases

• Faster equilibration between runs

• Shorter retention for many compounds

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Balanced speed and resolution

Typical Applications

• USP L10 methods

• Polar compound analysis

• Neutral compound analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Method development

• Quality control testing

• Routine analytical testing

• Alternative selectivity studies

• Stability testing

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Cyano™

• USP Classification: L10

• Chromatography Mode: Reversed Phase

• Bonded Phase: Cyano (CN)

• Endcapping: Not End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 4.0 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Special Order Item Information

This is a custom manufactured item that cannot be canceled or returned once an order has been placed. Please review the Special Order Item Information for complete details.

For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for reversed-phase HPLC applications

• Provides alternative selectivity compared to C18, C8, and Phenyl phases

• Suitable for polar and neutral compounds

• Faster equilibration than many traditional reversed-phase columns

• The 100 mm length provides greater resolution than 50 mm columns while maintaining practical run times

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• Packed and shipped in reversed-phase solvents

• For normal-phase applications, view Cogent NP Cyano Columns packed in normal-phase solvents

• This is a Special Order Item