HPLC Column, RP Cyano, Wide Pore, 3um, 4.0mm ID x 100mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP Cyano™ Wide Pore is a versatile reversed-phase HPLC stationary phase designed for the separation of larger polar and neutral compounds. Manufactured using a three-carbon ligand with a terminal cyano (CN) functional group bonded to ultra-high purity silica, this 300 Å wide pore stationary phase provides selectivity distinct from traditional C18, C8, and phenyl phases while offering greater accessibility for large analytes. As a USP L10 equivalent phase, it is particularly useful for proteins, peptides, enzymes, natural products, and other larger molecules that benefit from wide pore chromatography.
68334-10P-4-3M is an HPLC column packed with 3 µm RP Cyano™ Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 100 mm format. The 100 mm length provides greater chromatographic resolution than shorter 50 mm columns while maintaining practical analysis times for routine laboratory use. The wide pore structure improves analyte access to the bonded phase for larger compounds, while the 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.
This column is particularly useful for large polar or neutral compounds including proteins, peptides, enzymes, and natural products analyzed by reversed-phase HPLC. Compared to non-modified silica columns, the RP Cyano™ Wide Pore phase typically provides shorter retention times and faster equilibration between runs. The 100 mm format offers an excellent balance between resolution and analysis speed for bioanalytical, pharmaceutical, and research applications.
Key Benefits
RP Cyano™ Wide Pore stationary phase
USP L10 equivalent chemistry
Cyano bonded phase
Ultra-high purity silica support
300 Å wide pore design
Optimized for large molecules
Alternative selectivity to C18 and C8 phases
Faster equilibration between runs
3 µm particle size
Enhanced resolution for larger analytes
Typical Applications
USP L10 methods
Protein analysis
Peptide analysis
Enzyme analysis
Natural product analysis
Polar compound analysis
Neutral compound analysis
Bioanalytical methods
Reversed-phase HPLC
Pharmaceutical analysis
Specifications
Product Type: HPLC Column
Stationary Phase: RP Cyano™ Wide Pore
USP Classification: L10
Chromatography Mode: Reversed Phase
Bonded Phase: Cyano (CN)
Endcapping: Not End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 100 mm
Length: 100 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
This is a custom manufactured item that cannot be canceled or returned once an order has been placed. Please review the Special Order Item Information for complete details.
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Designed for larger molecules including proteins, peptides, enzymes, and natural products
300 Å wide pores improve access to the bonded phase for large analytes
Provides alternative selectivity compared to C18, C8, and Phenyl phases
Suitable for reversed-phase HPLC applications
Faster equilibration than many traditional reversed-phase columns
The 100 mm length provides greater resolution than 50 mm columns while maintaining practical run times
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
The wide pore design is well suited for biomolecules and larger analytes
Packed and shipped in reversed-phase solvents
For normal-phase applications, view the related Cogent NP Cyano Columns packed in normal-phase solvents
This is a Special Order Item