HPLC Column, RP Cyano, Wide Pore, 3um, 4.0mm ID x 250mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP Cyano™ Wide Pore is a versatile reversed-phase HPLC stationary phase designed for the separation of larger polar and neutral compounds. Manufactured using a three-carbon ligand with a terminal cyano (CN) functional group bonded to ultra-high purity silica, this 300 Å wide pore stationary phase provides selectivity distinct from traditional C18, C8, and phenyl phases while offering increased accessibility for large analytes. As a USP L10 equivalent phase, it is particularly useful for proteins, peptides, enzymes, natural products, and other larger molecules that benefit from wide pore chromatography.
68334-25P-4-3M is an HPLC column packed with 3 µm RP Cyano™ Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 250 mm format. The 250 mm length provides maximum chromatographic resolution and peak capacity within the RP Cyano product family, while the wide pore structure improves analyte access to the bonded phase for larger compounds. The 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.
This column is particularly useful for large polar or neutral compounds including proteins, peptides, enzymes, and natural products analyzed by reversed-phase HPLC. Compared to non-modified silica columns, the RP Cyano™ Wide Pore phase typically provides shorter retention times and faster equilibration between runs. The extended 250 mm format delivers maximum resolving power for challenging separations involving biomolecules, complex sample matrices, and applications requiring exceptional chromatographic resolution.
Key Benefits
RP Cyano™ Wide Pore stationary phase
USP L10 equivalent chemistry
Cyano bonded phase
Ultra-high purity silica support
300 Å wide pore design
Optimized for large molecules
Alternative selectivity to C18 and C8 phases
Faster equilibration between runs
3 µm particle size
Maximum chromatographic resolution
Typical Applications
USP L10 methods
Protein analysis
Peptide analysis
Enzyme analysis
Natural product analysis
Polar compound analysis
Neutral compound analysis
Bioanalytical methods
Reversed-phase HPLC
Pharmaceutical analysis
Specifications
Product Type: HPLC Column
Stationary Phase: RP Cyano™ Wide Pore
USP Classification: L10
Chromatography Mode: Reversed Phase
Bonded Phase: Cyano (CN)
Endcapping: Not End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
Please review the Special Order Item Information for complete details.
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Designed for larger molecules including proteins, peptides, enzymes, and natural products
300 Å wide pores improve access to the bonded phase for large analytes
Provides alternative selectivity compared to C18, C8, and Phenyl phases
Suitable for reversed-phase HPLC applications
Faster equilibration than many traditional reversed-phase columns
The 250 mm length provides maximum resolution and peak capacity for demanding separations
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
The wide pore design is optimized for biomolecules and larger analytes
Particularly useful for complex biological samples and high-resolution separations
Packed and shipped in reversed-phase solvents
For normal-phase applications, view the related Cogent NP Cyano Columns packed in Normal Phase solvents
This is a Special Order Item