Catalog Number - 68339-10P

Cogent RP Phenyl Hexyl™ is a reversed-phase HPLC stationary phase designed to provide enhanced aromatic selectivity through strong π-π interactions while maintaining hydrophobic retention characteristics. Manufactured using a six-carbon linker with a terminal phenyl ring bonded to ultra-high purity silica and fully double end capped, this stationary phase differs from traditional Phenyl phases by positioning the aromatic functionality farther from the silica surface. This increased distance reduces silanol influence and often enhances aromatic interactions, making the phase especially useful for compounds containing benzene rings and other aromatic structures. It is commonly used as an alternative in USP methods that specify L11 chemistry or Phenyl Hexyl phases.

68339-10P is an HPLC column packed with 3 µm RP Phenyl Hexyl™ particles featuring a 100 Å pore size in a 4.6 mm ID x 100 mm format. The 100 mm length offers an excellent balance between chromatographic resolution and analysis speed, making it suitable for both routine and advanced analytical methods. The 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.

This column is especially useful for the separation of aromatic compounds with moderate hydrophobicity, where the extended C6 linker can provide selectivity differences compared to traditional Phenyl phases. Typical applications include positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. For maximizing aromatic π-π interactions, methanol is often preferred over acetonitrile as the organic modifier.

Key Benefits

• RP Phenyl Hexyl™ stationary phase

• USP L11 equivalent selectivity

• Fully double end capped

• Six-carbon linker with terminal phenyl functionality

• Ultra-high purity silica support

• Enhanced π-π interactions

• Improved aromatic compound selectivity

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Balanced speed and resolution

Typical Applications

• USP L11 methods

• Aromatic compound analysis

• Positional isomer separations

• Tocopherol analysis

• Flavonoid analysis

• Polynuclear aromatic hydrocarbon analysis

• Nitro-aromatic compound analysis

• Active pharmaceutical ingredient (API) analysis

• Natural product analysis

• Method development

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Phenyl Hexyl™

• USP Classification: L11

• Chromatography Mode: Reversed Phase

• Bonded Phase: Phenyl Hexyl

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• Designed for aromatic compounds and compounds containing benzene rings

• The six-carbon linker positions the phenyl group farther from the silica surface, enhancing aromatic interactions

• Provides selectivity distinct from traditional Phenyl phases

• Suitable for reversed-phase HPLC applications

• The 100 mm length provides greater resolution than 50 mm columns while maintaining practical run times

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• Methanol is often preferred over acetonitrile when optimizing π-π interactions

• Particularly useful for APIs, natural products, flavonoids, and aromatic isomer separations

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