HPLC Column, RP Phenyl Hexyl, 3um, 4.6mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Phenyl Hexyl™ is a reversed-phase HPLC stationary phase designed to provide enhanced aromatic selectivity through strong π-π interactions while maintaining hydrophobic retention characteristics. Manufactured using a six-carbon linker with a terminal phenyl ring bonded to ultra-high purity silica and fully double end capped, this stationary phase differs from traditional Phenyl phases by positioning the aromatic functionality farther from the silica surface. This increased distance reduces silanol influence and often enhances aromatic interactions, making the phase especially useful for compounds containing benzene rings and other aromatic structures. It is commonly used as an alternative in USP methods that specify L11 chemistry or Phenyl Hexyl phases.
68339-25P is an HPLC column packed with 3 µm RP Phenyl Hexyl™ particles featuring a 100 Å pore size in a 4.6 mm ID x 250 mm format. The 250 mm length provides maximum chromatographic resolution and peak capacity within the RP Phenyl Hexyl product family, while the 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.
This column is especially useful for the separation of aromatic compounds with moderate hydrophobicity, where the extended C6 linker can provide selectivity differences compared to traditional Phenyl phases. Typical applications include positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. The 250 mm format provides exceptional resolving power for challenging separations, impurity profiling, and complex sample matrices. For maximizing aromatic π-π interactions, methanol is often preferred over acetonitrile as the organic modifier.
Key Benefits
RP Phenyl Hexyl™ stationary phase
USP L11 equivalent selectivity
Fully double end capped
Six-carbon linker with terminal phenyl functionality
Ultra-high purity silica support
Enhanced π-π interactions
Improved aromatic compound selectivity
3 µm particle size
Greater efficiency than 5 µm columns
Maximum chromatographic resolution
Typical Applications
USP L11 methods
Aromatic compound analysis
Positional isomer separations
Tocopherol analysis
Flavonoid analysis
Polynuclear aromatic hydrocarbon analysis
Nitro-aromatic compound analysis
Active pharmaceutical ingredient (API) analysis
Natural product analysis
Impurity profiling
Specifications
Product Type: HPLC Column
Stationary Phase: RP Phenyl Hexyl™
USP Classification: L11
Chromatography Mode: Reversed Phase
Bonded Phase: Phenyl Hexyl
Endcapping: Fully Double End Capped
Particle Size: 3 µm
Pore Size: 100 Å
Column Dimensions: 4.6 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Important Notes
Designed for aromatic compounds and compounds containing benzene rings
The six-carbon linker positions the phenyl group farther from the silica surface, enhancing aromatic interactions
Provides selectivity distinct from traditional Phenyl phases
Suitable for reversed-phase HPLC applications
The 250 mm length provides maximum resolution and peak capacity for demanding separations
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Generates higher backpressure than larger particle columns
Methanol is often preferred over acetonitrile when optimizing π-π interactions
Particularly useful for APIs, natural products, flavonoids, aromatic isomers, and impurity profiling studies