Catalog Number - 68508-25P-4

Cogent RP C8™ HPLC Columns are designed for reliable reversed-phase chromatography using a traditional octyl (C8) bonded stationary phase. Manufactured with ultra-high purity silica and fully double end capped bonding technology, these columns provide reproducible retention, excellent peak shape, and long column life. The RP C8™ phase is a USP L7 equivalent chemistry and is commonly used as an alternative for methods developed on similar C8 phases. Compared to C18 columns, RP C8™ generally provides lower hydrophobic retention while maintaining excellent selectivity for many neutral and non-polar compounds.

68508-25P-4 is packed with 5 µm RP C8™ particles featuring a 100 Å pore size in a 4.0 mm ID x 250 mm length format. The 250 mm column length provides maximum resolving power and increased peak capacity for complex analytical separations where the highest chromatographic performance is required. The RP C8™ stationary phase is particularly useful for hydrophobic compounds, neutral analytes, and long-chain fatty acids where somewhat lower retention than a comparable C18 column is preferred. The 5 µm particle size provides low backpressure and dependable chromatographic performance on conventional HPLC systems.

This column is frequently selected as a starting point for new method development and is also well suited as a replacement for methods specifying USP L7 chemistry. The 4.0 mm internal diameter offers an effective balance between sensitivity, solvent consumption, and compatibility with traditional analytical HPLC instrumentation. This configuration is ideal for pharmaceutical, environmental, food, and routine analytical applications requiring robust performance and the highest practical analytical resolution.

Key Benefits

• RP C8™ stationary phase

• USP L7 equivalent chemistry

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 100 Å pore size

• Maximum analytical resolution

• Increased peak capacity

• Lower retention than C18 phases

• Excellent for method development and USP method replacement

Typical Applications

• Reversed-phase HPLC

• USP L7 method substitution

• Method development

• Method validation

• Pharmaceutical analysis

• Long-chain fatty acid analysis

• Hydrophobic compound separations

• Neutral compound analysis

• Environmental testing

• Quality control testing

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8™

• USP Classification: L7

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 4.0 mm ID x 250 mm

• Recommended Flow Rate: 0.8-1.5 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Special Order Item Information

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for high-resolution reversed-phase HPLC separations

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Excellent choice for long-chain fatty acid separations

• Frequently selected for USP L7 method replacement and transfer

• 250 mm length provides maximum resolving power and peak capacity

• 5 µm particles provide low backpressure and broad instrument compatibility

• Compatible with standard HPLC systems operating at conventional analytical flow rates

• Commonly used as a starting column for new analytical method development

• Manufactured using ultra-high purity silica and fully double end capped bonding technology

• This is a Special Order Item

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