HPLC Column, RP C8 Octyl, Wide Pore, 5um, 4.0mm ID x 250mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP C8™ Wide Pore HPLC Columns are designed for reversed-phase separations of larger molecules that require enhanced pore accessibility and efficient mass transfer. The stationary phase consists of a traditional octyl (C8) bonded ligand attached to ultra-high purity silica and is fully double end capped to minimize secondary interactions while providing reproducible retention, excellent peak shape, and long column life. The 300 Å wide pore structure allows proteins, peptides, and other higher molecular weight compounds to access more of the bonded surface area, making this phase particularly suitable for biomolecule analyses and challenging large-molecule separations.
68508-25P-4-3M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 250 mm length format. The 250 mm column length provides maximum resolving power and increased peak capacity for complex analytical separations involving proteins, peptides, and other larger molecules. The wide pore architecture improves accessibility and mass transfer for biomolecules and long-chain fatty acids that may be partially excluded from conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for hydrophobic and moderately non-polar compounds.
This column is frequently selected as a starting point for new method development involving larger molecules and is also suitable as a replacement for USP L7 methods where wide pore media may provide analytical advantages. The 4.0 mm internal diameter offers an effective balance between sensitivity, solvent consumption, and compatibility with conventional analytical HPLC instrumentation. This configuration is well suited for pharmaceutical, biopharmaceutical, protein, peptide, and biomolecule applications requiring the highest practical analytical resolution.
Key Benefits
RP C8™ Wide Pore stationary phase
Octyl (C8) bonded phase
Fully double end capped
Ultra-high purity silica support
300 Å wide pore design
Ideal for proteins and peptides
Maximum analytical resolution
Increased peak capacity
Lower retention than C18 phases
Excellent for method development and large molecule separations
Typical Applications
Reversed-phase HPLC
Protein analysis
Peptide analysis
Biomolecule separations
Long-chain fatty acid analysis
Pharmaceutical analysis
Biopharmaceutical analysis
Method development
Method validation
High-resolution analytical separations
Specifications
Product Type: HPLC Column
Stationary Phase: RP C8™ Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Octyl (C8)
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 250 mm
Recommended Flow Rate: 0.8-1.5 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Special Order Item Information
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Designed for large molecule reversed-phase separations
300 Å pores enhance accessibility for proteins, peptides, and biomolecules
Provides lower retention than comparable C18 columns
Suitable for hydrophobic and moderately non-polar compounds
Excellent choice for protein, peptide, biomolecule, and long-chain fatty acid analyses
250 mm length provides maximum resolving power and peak capacity
5 µm particles provide low backpressure and broad instrument compatibility
Compatible with standard HPLC systems operating at conventional analytical flow rates
Frequently selected for method development, method validation, and USP L7 method replacement involving larger molecules
Manufactured using ultra-high purity silica and fully double end capped bonding technology
This is a Special Order Item