Catalog Number - 68508-P21-250

Cogent RP C8™ Preparative HPLC Columns are designed for purification and scale-up applications using a traditional octyl (C8) bonded stationary phase. Manufactured with ultra-high purity silica and fully double end capped bonding technology, these columns provide reproducible retention, excellent peak shape, and long service life under preparative operating conditions. The RP C8™ phase offers lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for hydrophobic and neutral compounds commonly encountered in purification workflows.

68508-P21-250 is packed with 5 µm RP C8™ particles featuring a 100 Å pore size in a 21.2 mm ID x 250 mm length preparative format. The extended column length provides maximum resolving power and increased loading capacity for demanding purification applications. The large internal diameter supports substantial sample loading for compound isolation, fraction collection, and preparative method development. The RP C8™ stationary phase is particularly useful for hydrophobic compounds, neutral analytes, and long-chain fatty acids where lower retention than a comparable C18 phase is desired.

The 21.2 mm ID x 250 mm format is commonly selected when maximum purification efficiency and resolution are required. Typical flow rates are approximately 15-25 mL/min depending on mobile phase composition and system configuration. A specialized preparative HPLC system capable of delivering higher flow rates, larger injection volumes, and increased sample loading is required for optimum performance.

Key Benefits

• RP C8™ stationary phase

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 100 Å pore size

• High sample loading capacity

• Maximum preparative resolution

• Lower retention than C18 phases

• Designed for purification workflows

• Suitable for scale-up applications

Typical Applications

• Preparative HPLC

• Compound purification

• Fraction collection

• Method scale-up

• Pharmaceutical purification

• Natural product isolation

• Long-chain fatty acid purification

• Hydrophobic compound purification

• Process development

• Research and development applications

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: RP C8™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 21.2 mm ID x 250 mm

• Recommended Flow Rate: 15-25 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for preparative reversed-phase HPLC purification

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Excellent choice for compound isolation and fraction collection

• 21.2 mm ID format provides substantially increased sample loading capacity

• 250 mm length provides maximum resolving power and purification efficiency

• 5 µm particles support preparative flow rates with manageable backpressure

• Typical operating range is approximately 15-25 mL/min

• Requires a specialized preparative HPLC system capable of higher flow rates and sample loads

• Suitable for analytical-to-preparative method scale-up

• This is a Special Order Item