Catalog Number - 68508-P21-2503M

Cogent RP C8™ Wide Pore Preparative HPLC Columns are designed for purification and scale-up applications involving larger molecules that require enhanced pore accessibility. The stationary phase consists of a traditional octyl (C8) bonded ligand attached to ultra-high purity silica and is fully double end capped to minimize secondary interactions while providing reproducible retention and excellent chromatographic performance. The 300 Å wide pore structure allows proteins, peptides, and other higher molecular weight compounds to access more of the bonded surface area, making this phase particularly well suited for biomolecule purification.

68508-P21-2503M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 21.2 mm ID x 250 mm length preparative format. The extended column length provides maximum resolving power and increased loading capacity for demanding purification applications involving proteins, peptides, and other large compounds. The wide pore architecture improves accessibility and mass transfer for biomolecules that may be partially excluded from conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for a broad range of hydrophobic and neutral analytes.

The 21.2 mm ID x 250 mm format is commonly selected when maximum purification efficiency and resolution are required during analytical-to-preparative scale-up. Typical flow rates are approximately 15-25 mL/min depending on mobile phase composition and system configuration. A specialized preparative HPLC system capable of delivering higher flow rates, larger injection volumes, and increased sample loading is required for optimum performance.

Key Benefits

• RP C8™ Wide Pore stationary phase

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 300 Å wide pore design

• High sample loading capacity

• Ideal for proteins and peptides

• Maximum preparative resolution

• Lower retention than C18 phases

• Suitable for scale-up applications

Typical Applications

• Preparative HPLC

• Protein purification

• Peptide purification

• Biomolecule purification

• Fraction collection

• Method scale-up

• Pharmaceutical purification

• Biopharmaceutical purification

• Process development

• Compound isolation

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: RP C8™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 21.2 mm ID x 250 mm

• Recommended Flow Rate: 15-25 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for preparative purification of proteins, peptides, and other biomolecules

• 300 Å pores improve accessibility for larger molecular weight compounds

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• High sample loading capacity supports efficient purification workflows

• 250 mm length provides maximum resolving power and purification efficiency

• 5 µm particles support preparative flow rates with manageable backpressure

• Typical operating range is approximately 15-25 mL/min

• Requires a specialized preparative HPLC system capable of higher flow rates and sample loads

• Suitable for analytical-to-preparative method scale-up

• This is a Special Order Item