Catalog Number - 68508-P30-50-3M

Cogent RP C8™ Wide Pore Preparative HPLC Columns are designed for purification and scale-up applications involving larger molecules that require enhanced pore accessibility. The stationary phase consists of a traditional octyl (C8) bonded ligand attached to ultra-high purity silica and is fully double end capped to minimize secondary interactions while providing reproducible retention and excellent chromatographic performance. The 300 Å wide pore structure allows proteins, peptides, and other higher molecular weight compounds to access more of the bonded surface area, making this phase particularly well suited for biomolecule purification and preparative separations.

68508-P30-50-3M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 30 mm ID x 50 mm length preparative format. The extra-large internal diameter provides very high sample loading capacity while the shorter column length minimizes run times and solvent consumption. The wide pore architecture improves accessibility and mass transfer for proteins, peptides, and other large compounds that may be partially excluded from conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for a broad range of biomolecules and hydrophobic compounds.

The 30 mm ID format is commonly used for large-scale preparative purification, rapid purification screening, and analytical-to-preparative method scale-up. Typical flow rates are approximately 30-50 mL/min depending on mobile phase composition and system configuration. A specialized preparative HPLC system capable of delivering high flow rates, large injection volumes, and substantial sample loading is required for optimum performance.

Key Benefits

• RP C8™ Wide Pore stationary phase

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 300 Å wide pore design

• Very high sample loading capacity

• Ideal for proteins and peptides

• Lower retention than C18 phases

• Fast preparative run times

• Suitable for scale-up applications

Typical Applications

• Preparative HPLC

• Protein purification

• Peptide purification

• Biomolecule purification

• Fraction collection

• Method scouting

• Method scale-up

• Pharmaceutical purification

• Biopharmaceutical purification

• Process development

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: RP C8™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 30 mm ID x 50 mm

• Recommended Flow Rate: 30-50 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for preparative purification of proteins, peptides, and other biomolecules

• 300 Å pores improve accessibility for larger molecular weight compounds

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Very high sample loading capacity supports efficient purification workflows

• Short 50 mm length minimizes run times and solvent consumption

• 5 µm particles support preparative flow rates with manageable backpressure

• Typical operating range is approximately 30-50 mL/min

• Requires a specialized preparative HPLC system capable of high flow rates and sample loads

• Suitable for analytical-to-preparative method scale-up

• This is a Special Order Item

  ---