Catalog Number - 68508-P50-1503M

Cogent RP C8™ Wide Pore Process HPLC Columns are designed for large-scale purification and process chromatography applications involving larger molecules that require enhanced pore accessibility. The stationary phase consists of a traditional octyl (C8) bonded ligand attached to ultra-high purity 300 Å silica and is fully double end capped to minimize secondary interactions while providing reproducible retention and excellent chromatographic performance. The 300 Å wide pore structure allows proteins, peptides, and other higher molecular weight compounds to fully access the bonded surface area, making this phase particularly well suited for biomolecule purification and process-scale separations.

68508-P50-1503M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 50 mm ID x 150 mm length process-scale format. The increased column length provides greater resolving power and purification efficiency, while the large internal diameter delivers exceptionally high sample loading capacity for demanding production-scale separations. The wide pore architecture improves accessibility and mass transfer for proteins, peptides, and other large compounds that may be partially excluded from conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for a broad range of biomolecules and hydrophobic compounds.

The 50 mm ID format is intended for process chromatography, pilot production, and manufacturing-scale purification operations. Typical flow rates are approximately 80-140 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized process HPLC system capable of high flow rates, large injection volumes, and substantial sample loading is required for optimum performance.

Key Benefits

• RP C8™ Wide Pore stationary phase

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity 300 Å silica

• 300 Å wide pore design

• Exceptionally high sample loading capacity

• Ideal for proteins and peptides

• Lower retention than C18 phases

• Increased purification efficiency

• Supports high flow rate operation

Typical Applications

• Process HPLC

• Protein purification

• Peptide purification

• Biomolecule purification

• Production-scale separations

• Fraction collection

• Pharmaceutical purification

• Biopharmaceutical purification

• Process development

• Large-scale chromatography

Specifications

• Product Type: Process HPLC Column

• Stationary Phase: RP C8™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 50 mm ID x 150 mm

• Recommended Flow Rate: 80-140 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for process-scale purification of proteins, peptides, and other biomolecules

• 300 Å pores improve accessibility for larger molecular weight compounds

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Exceptionally high sample loading capacity supports efficient purification workflows

• 50 mm ID format is ideal for pilot plant and production-scale chromatography

• 150 mm length provides improved resolution and purification efficiency

• 5 µm particles support high flow rates with manageable backpressure

• Typical operating range is approximately 80-140 mL/min

• Requires a specialized process HPLC system capable of high flow rates and substantial sample loading

• Suitable for preparative-to-process method scale-up

• This is a Special Order Item