Process HPLC Column, RP C8 Octyl, Wide Pore, 5um, 300A. 50mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C8™ Wide Pore Process HPLC Columns are designed for large-scale purification and process chromatography applications involving larger molecules that require enhanced pore accessibility. The stationary phase consists of a traditional octyl (C8) bonded ligand attached to ultra-high purity 300 Å silica and is fully double end capped to minimize secondary interactions while providing reproducible retention and excellent chromatographic performance. The 300 Å wide pore structure allows proteins, peptides, and other higher molecular weight compounds to fully access the bonded surface area, making this phase particularly well suited for biomolecule purification and process-scale separations.
68508-P50-50-3M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 50 mm ID x 50 mm length process-scale format. The large internal diameter provides exceptionally high sample loading capacity while the shorter column length minimizes run times and solvent consumption. The wide pore architecture improves accessibility and mass transfer for proteins, peptides, and other large compounds that may be partially excluded from conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for a broad range of biomolecules and hydrophobic compounds.
The 50 mm ID format is intended for process chromatography, pilot production, rapid purification screening, and manufacturing-scale purification operations. Typical flow rates are approximately 80-140 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized process HPLC system capable of high flow rates, large injection volumes, and substantial sample loading is required for optimum performance.
Key Benefits
RP C8™ Wide Pore stationary phase
Octyl (C8) bonded phase
Fully double end capped
Ultra-high purity 300 Å silica
300 Å wide pore design
Exceptionally high sample loading capacity
Ideal for proteins and peptides
Lower retention than C18 phases
Fast process-scale run times
Supports high flow rate operation
Typical Applications
Process HPLC
Protein purification
Peptide purification
Biomolecule purification
Production-scale separations
Fraction collection
Pharmaceutical purification
Biopharmaceutical purification
Process development
Large-scale chromatography
Specifications
Product Type: Process HPLC Column
Stationary Phase: RP C8™ Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Octyl (C8)
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 50 mm ID x 50 mm
Recommended Flow Rate: 80-140 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Special Order Item Information
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Important Notes
Designed for process-scale purification of proteins, peptides, and other biomolecules
300 Å pores improve accessibility for larger molecular weight compounds
Provides lower retention than comparable C18 columns
Suitable for hydrophobic and moderately non-polar compounds
Exceptionally high sample loading capacity supports efficient purification workflows
Short 50 mm length minimizes run times and solvent consumption
50 mm ID format is ideal for pilot plant and production-scale chromatography
5 µm particles support high flow rates with manageable backpressure
Typical operating range is approximately 80-140 mL/min
Requires a specialized process HPLC system capable of high flow rates and substantial sample loading
This is a Special Order Item