Catalog Number - 68508-SP250-3M

Cogent RP C8™ Wide Pore Semi-Preparative HPLC Columns are designed for purification, method scale-up, and high-capacity separations involving larger molecules that require enhanced pore accessibility. The stationary phase consists of a traditional octyl (C8) bonded ligand attached to ultra-high purity 300 Å silica and is fully double end capped to minimize secondary interactions while providing reproducible retention, excellent peak shape, and long service life. The 300 Å wide pore structure allows proteins, peptides, and other higher molecular weight compounds to fully access the bonded surface area, making this phase particularly well suited for biomolecule purification and semi-preparative separations.

68508-SP250-3M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 10 mm ID x 250 mm length semi-preparative format. The extended column length provides maximum resolving power and purification efficiency while maintaining substantially higher sample loading capacity than analytical columns. The wide pore architecture improves accessibility and mass transfer for proteins, peptides, and other large compounds that may be partially excluded from conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for large hydrophobic and neutral compounds.

The 10 mm ID format bridges the gap between analytical and preparative chromatography, making it ideal for purification projects requiring increased sample throughput without moving to full preparative systems. Typical flow rates are approximately 4-8 mL/min depending on mobile phase composition and system configuration. A semi-preparative or preparative HPLC system capable of higher flow rates and larger injection volumes is recommended for optimum performance.

Key Benefits

• RP C8™ Wide Pore stationary phase

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity 300 Å silica

• 300 Å wide pore design

• Increased sample loading capacity

• Ideal for proteins and peptides

• Maximum semi-preparative resolution

• Lower retention than C18 phases

• Designed for purification workflows

Typical Applications

• Semi-preparative HPLC

• Protein purification

• Peptide purification

• Biomolecule purification

• Fraction collection

• Method scale-up

• Pharmaceutical purification

• Biopharmaceutical purification

• Process development

• Large molecule separations

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: RP C8™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 10 mm ID x 250 mm

• Recommended Flow Rate: 4-8 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for semi-preparative purification of proteins, peptides, and other biomolecules

• 300 Å pores improve accessibility for larger molecular weight compounds

• Suitable for large hydrophobic and neutral compounds

• Provides lower retention than comparable C18 columns

• 10 mm ID format provides increased sample loading capacity compared to analytical columns

• 250 mm length provides maximum resolving power and purification efficiency

• 5 µm particles support semi-preparative flow rates with manageable backpressure

• Typical operating range is approximately 4-8 mL/min

• Requires a semi-preparative or preparative HPLC system capable of higher flow rates and injection volumes

• Suitable for analytical-to-semi-preparative method scale-up

• This is a Special Order Item