Catalog Number - 68508-SP50

Cogent RP C8™ Semi-Preparative HPLC Columns are designed for purification, method scale-up, and high-capacity separations using a traditional octyl (C8) bonded stationary phase. Manufactured with ultra-high purity silica and fully double end capped bonding technology, these columns provide reproducible retention, excellent peak shape, and long service life. The RP C8™ phase offers lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for hydrophobic and neutral compounds commonly encountered in purification workflows.

68508-SP50 is packed with 5 µm RP C8™ particles featuring a 100 Å pore size in a 10 mm ID x 50 mm length semi-preparative format. The larger internal diameter provides substantially increased sample loading capacity compared to analytical columns, while the shorter column length reduces run times and solvent consumption. This configuration is ideal for rapid purification, method scouting, fraction collection, and analytical-to-semi-preparative method transfer. The RP C8™ stationary phase is particularly useful for hydrophobic compounds, neutral analytes, and long-chain fatty acids where lower retention than a comparable C18 phase is desired.

The 10 mm ID format bridges the gap between analytical and preparative chromatography, making it ideal for purification projects requiring greater sample throughput without moving to full preparative systems. Typical flow rates are approximately 4-8 mL/min depending on mobile phase composition and system configuration. A semi-preparative or preparative HPLC system capable of higher flow rates and larger injection volumes is recommended for optimum performance.

Key Benefits

• RP C8™ stationary phase

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 100 Å pore size

• Increased sample loading capacity

• Lower retention than C18 phases

• Fast purification run times

• Ideal for semi-preparative purification

• Suitable for method scale-up

Typical Applications

• Semi-preparative HPLC

• Compound purification

• Fraction collection

• Method scouting

• Method scale-up

• Pharmaceutical purification

• Natural product isolation

• Long-chain fatty acid purification

• Hydrophobic compound separations

• Process development

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: RP C8™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 10 mm ID x 50 mm

• Recommended Flow Rate: 4-8 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Special Order Item Information

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for semi-preparative reversed-phase purification

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Excellent choice for compound isolation and fraction collection

• 10 mm ID format provides increased sample loading capacity compared to analytical columns

• Short 50 mm length minimizes run times and solvent consumption

• 5 µm particles support semi-preparative flow rates with manageable backpressure

• Typical operating range is approximately 4-8 mL/min

• Requires a semi-preparative or preparative HPLC system capable of higher flow rates and injection volumes

• Suitable for analytical-to-semi-preparative method scale-up

• This is a Special Order Item