HPLC Column, RP C12, 5um, 2.1mm ID x 150mm Length, 100A. Cogent Brand. 1 EA.

Additional Information:

Cogent RP C12™ HPLC Columns are designed for reversed-phase separations requiring unique selectivity, rapid equilibration, and reproducible retention. The stationary phase is manufactured using a rigid adamantane-shaped carbon silane ligand bonded to ultra-high purity silica and fully double end capped to minimize secondary interactions. This distinctive bonded phase chemistry offers chromatographic behavior between traditional C8 and C18 phases, making it especially useful for complex mixtures, analytical method development, and applications requiring alternative selectivity.

68512-15P-2 is packed with 5 µm RP C12™ particles featuring a 100 Å pore size in a 2.1 mm ID x 150 mm format. The narrow-bore internal diameter reduces solvent consumption and enhances compatibility with LC-MS systems, while the 150 mm length provides increased resolution and peak capacity for complex separations. The RP C12™ phase typically provides less retention than C18 columns but greater retention than C8 columns for many hydrophobic compounds, offering a valuable alternative selectivity profile for challenging analytes. The stationary phase is also compatible with highly aqueous mobile phases, including 100% water, without phase collapse or loss of retention.

The 2.1 mm ID x 150 mm format is well suited for LC-MS applications, pharmaceutical analysis, complex mixture separations, and advanced method development. Typical flow rates are approximately 0.2-0.3 mL/min. Standard HPLC systems used with 2.1 mm ID columns must be capable of delivering stable and reproducible low flow rates, and detector flow cells should be optimized for low-volume chromatography to achieve maximum performance.

Key Benefits

  • RP C12™ stationary phase

  • Adamantane-shaped bonded ligand

  • Fully double end capped

  • Ultra-high purity silica support

  • 100 Å pore size

  • Unique selectivity profile

  • Compatible with 100% aqueous mobile phases

  • Rapid column equilibration

  • Enhanced chromatographic resolution

  • Excellent for LC-MS applications

Typical Applications

  • LC-MS methods

  • LC-MS/MS analysis

  • Reversed-phase HPLC

  • Pharmaceutical analysis

  • Method development

  • Complex mixture separations

  • Quality control testing

  • Research and development

  • Hydrophobic compound analysis

  • Alternative selectivity studies

Specifications

  • Product Type: HPLC Column

  • Stationary Phase: RP C12™

  • Chromatography Mode: Reversed Phase

  • Bonded Phase: Adamantane-Shaped C12 Ligand

  • Endcapping: Fully Double End Capped

  • Particle Size: 5 µm

  • Pore Size: 100 Å

  • Column Dimensions: 2.1 mm ID x 150 mm

  • Recommended Flow Rate: 0.2-0.3 mL/min

  • Column Hardware: Stainless Steel

  • Brand: Cogent

Important Notes

  • Provides a selectivity profile between traditional C8 and C18 phases

  • Compatible with highly aqueous and 100% water mobile phases

  • Rapid equilibration minimizes time between injections

  • 150 mm length provides increased resolution and peak capacity

  • Excellent choice for LC-MS and LC-MS/MS methods

  • Useful for complex mixtures requiring alternative selectivity

  • 2.1 mm ID format minimizes solvent consumption and improves MS compatibility

  • Requires an HPLC system capable of reliable low-flow operation at approximately 0.2-0.3 mL/min

  • Best performance is achieved using low-volume detector flow cells and LC-MS interfaces

  • Suitable for advanced method development and challenging separations


 

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