Catalog Number - 68512-25P-4

Cogent RP C12™ HPLC Columns are designed for reversed-phase separations requiring unique selectivity, rapid equilibration, and reproducible retention. The stationary phase is manufactured using a rigid adamantane-shaped carbon silane ligand bonded to ultra-high purity silica and fully double end capped to minimize secondary interactions. This distinctive bonded phase chemistry offers chromatographic behavior between traditional C8 and C18 phases, making it especially useful for complex mixtures, analytical method development, and high-throughput screening applications.

68512-25P-4 is packed with 5 µm RP C12™ particles featuring a 100 Å pore size in a 4.0 mm ID x 250 mm format. The extended column length provides maximum resolving power and peak capacity, making it ideal for demanding analytical separations requiring enhanced resolution. The RP C12™ phase typically provides less retention than C18 columns but greater retention than C8 columns for many hydrophobic compounds, offering an alternative selectivity profile that can improve difficult chromatographic separations. The stationary phase is also compatible with highly aqueous mobile phases, including 100% water, without phase collapse or loss of retention.

The 4.0 mm ID x 250 mm format is commonly used for pharmaceutical analysis, quality control testing, method development, and complex sample separations where maximum chromatographic resolution is important. Typical flow rates are approximately 0.8-1.2 mL/min depending on mobile phase composition and method requirements.

Key Benefits

• RP C12™ stationary phase

• Adamantane-shaped bonded ligand

• Fully double end capped

• Ultra-high purity silica support

• 100 Å pore size

• Unique selectivity profile

• Compatible with 100% aqueous mobile phases

• Rapid column equilibration

• Maximum chromatographic resolution

• Ideal for complex separations

Typical Applications

• Reversed-phase HPLC

• Pharmaceutical analysis

• Method development

• Quality control testing

• Complex mixture separations

• High-throughput screening (HTS)

• Research and development

• Hydrophobic compound analysis

• Stability studies

• Routine analytical HPLC

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C12™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Adamantane-Shaped C12 Ligand

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 4.0 mm ID x 250 mm

• Recommended Flow Rate: 0.8-1.2 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Provides a selectivity profile between traditional C8 and C18 phases

• Compatible with highly aqueous and 100% water mobile phases

• Rapid equilibration minimizes time between injections

• 250 mm length provides maximum resolution and peak capacity

• Excellent choice for complex mixtures and challenging separations

• Useful for method development requiring alternative selectivity

• Suitable for routine analytical and quality control applications

• Popular for pharmaceutical analysis and research laboratories

• 4.0 mm ID format is compatible with most standard HPLC systems

• This is a Special Order Item