Catalog Number - 68512-P30-250

Cogent RP C12™ Preparative HPLC Columns are designed for purification, scale-up, and preparative chromatography applications requiring unique selectivity, rapid equilibration, and reproducible retention. The stationary phase is manufactured using a rigid adamantane-shaped carbon silane ligand bonded to ultra-high purity silica and fully double end capped to minimize secondary interactions. This distinctive bonded phase chemistry provides chromatographic behavior between traditional C8 and C18 phases, making it especially useful for preparative separations where alternative selectivity can improve compound purification.

68512-P30-250 is packed with 5 µm RP C12™ particles featuring a 100 Å pore size in a 30 mm ID x 250 mm length preparative format. The RP C12™ phase typically provides less retention than C18 columns while offering greater retention than C8 columns for many hydrophobic compounds. In addition, the stationary phase is compatible with highly aqueous reversed-phase mobile phases, including 100% water, without phase collapse or loss of retention.

The 30 mm ID x 250 mm format provides very high sample loading capacity and maximum purification efficiency for large-scale separations, fraction collection, process development, and production-oriented purification workflows. Typical flow rates are approximately 30–50 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized preparative HPLC system capable of high flow rates and large sample loading is required for optimum performance.

Key Benefits

• RP C12™ stationary phase

• Adamantane-shaped bonded ligand

• Fully double end capped

• Ultra-high purity silica support

• 100 Å pore size

• Unique selectivity profile

• Compatible with 100% aqueous mobile phases

• Rapid equilibration between runs

• Very high sample loading capacity

• Maximum preparative purification efficiency

Typical Applications

• Preparative HPLC

• Compound purification

• Fraction collection

• Method scale-up

• Pharmaceutical purification

• Natural product isolation

• Process development

• Production-scale chromatography

• Complex mixture separations

• Alternative selectivity applications

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: RP C12™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Adamantane-Shaped C12 Ligand

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 30 mm ID x 250 mm

• Recommended Flow Rate: 30–50 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information page for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for preparative reversed-phase purification

• Provides a selectivity profile between traditional C8 and C18 phases

• Compatible with highly aqueous and 100% water mobile phases

• Rapid equilibration minimizes preparation time between runs

• Suitable for hydrophobic and moderately non-polar compounds

• 30 mm ID format provides very high sample loading capacity

• 250 mm length provides maximum resolution and purification efficiency

• 5 µm particles support preparative flow rates with manageable backpressure

• Typical operating range is approximately 30–50 mL/min

• Requires a specialized preparative HPLC system capable of high flow rates and substantial sample loading

• Suitable for analytical-to-preparative method scale-up

• This is a Special Order Item.

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