Catalog Number - 68512-P50-250

Cogent RP C12™ Process HPLC Columns are designed for large-scale purification, process development, and production chromatography applications requiring unique selectivity, rapid equilibration, and reproducible retention. The stationary phase is manufactured using a rigid adamantane-shaped carbon silane ligand bonded to ultra-high purity silica and fully double end capped to minimize secondary interactions. This distinctive bonded phase chemistry provides chromatographic behavior between traditional C8 and C18 phases, making it especially useful for process separations where alternative selectivity can improve purification efficiency.

68512-P50-250 is packed with 5 µm RP C12™ particles featuring a 100 Å pore size in a 50 mm ID x 250 mm process-scale format. The extended column length provides maximum resolving power and purification efficiency while maintaining exceptionally high sample loading capacity for large-scale purification workflows. The RP C12™ phase typically provides less retention than C18 columns while offering greater retention than C8 columns for many hydrophobic compounds. In addition, the stationary phase is compatible with highly aqueous reversed-phase mobile phases, including 100% water, without phase collapse or loss of retention.

The 50 mm ID x 250 mm format is ideal for process chromatography, pilot production, in-process purification, and manufacturing-scale separations where maximum resolution and loading capacity are required. Typical flow rates are approximately 80-140 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized process HPLC system capable of high flow rates, large injection volumes, and substantial sample loading is required for optimum performance.

Key Benefits

• RP C12™ stationary phase

• Adamantane-shaped bonded ligand

• Fully double end capped

• Ultra-high purity silica support

• 100 Å pore size

• Unique selectivity profile

• Compatible with 100% aqueous mobile phases

• Rapid equilibration between runs

• Exceptionally high sample loading capacity

• Maximum purification efficiency

Typical Applications

• Process HPLC

• In-process purification

• Production-scale chromatography

• Compound purification

• Fraction collection

• Method scale-up

• Pharmaceutical purification

• Natural product isolation

• Process development

• Alternative selectivity applications

Specifications

• Product Type: Process HPLC Column

• Stationary Phase: RP C12™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Adamantane-Shaped C12 Ligand

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 50 mm ID x 250 mm

• Recommended Flow Rate: 80-140 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for process-scale reversed-phase purification

• Provides a selectivity profile between traditional C8 and C18 phases

• Compatible with highly aqueous and 100% water mobile phases

• Rapid equilibration minimizes preparation time between runs

• Suitable for hydrophobic and moderately non-polar compounds

• 50 mm ID format provides exceptionally high sample loading capacity

• 250 mm length provides maximum resolution and purification efficiency

• 5 µm particles support high flow rates with manageable backpressure

• Typical operating range is approximately 80-140 mL/min

• Requires a specialized process HPLC system capable of high flow rates and substantial sample loading

• Suitable for preparative-to-process method scale-up

• This is a Special Order Item