Catalog Number - 68512-SP50

Cogent RP C12™ Semi-Preparative HPLC Columns are designed for purification, method scale-up, and high-capacity separations requiring unique selectivity, rapid equilibration, and reproducible retention. The stationary phase is manufactured using a rigid adamantane-shaped carbon silane ligand bonded to ultra-high purity silica and fully double end capped to minimize secondary interactions. This distinctive bonded phase chemistry provides chromatographic behavior between traditional C8 and C18 phases, making it especially useful for purification workflows where alternative selectivity can improve compound isolation and recovery.

68512-SP50 is packed with 5 µm RP C12™ particles featuring a 100 Å pore size in a 10 mm ID x 50 mm length semi-preparative format. The larger internal diameter provides substantially increased sample loading capacity compared to analytical columns, while the shorter column length reduces run times and solvent consumption. The RP C12™ phase typically provides less retention than C18 columns while offering greater retention than C8 columns for many hydrophobic compounds. In addition, the stationary phase is compatible with highly aqueous reversed-phase mobile phases, including 100% water, without phase collapse or loss of retention.

The 10 mm ID x 50 mm format is ideal for rapid purification, method scouting, analytical-to-semi-preparative method scale-up, and fraction collection workflows. Typical flow rates are approximately 4-8 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A semi-preparative or preparative HPLC system capable of higher flow rates and larger injection volumes is recommended for optimum performance.

Key Benefits

• RP C12™ stationary phase

• Adamantane-shaped bonded ligand

• Fully double end capped

• Ultra-high purity silica support

• 100 Å pore size

• Unique selectivity profile

• Compatible with 100% aqueous mobile phases

• Rapid equilibration between runs

• Increased sample loading capacity

• Fast purification run times

Typical Applications

• Semi-preparative HPLC

• Compound purification

• Fraction collection

• Method scouting

• Method scale-up

• Pharmaceutical purification

• Natural product isolation

• Process development

• Complex mixture separations

• Alternative selectivity applications

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: RP C12™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Adamantane-Shaped C12 Ligand

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 10 mm ID x 50 mm

• Recommended Flow Rate: 4-8 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for semi-preparative reversed-phase purification

• Provides a selectivity profile between traditional C8 and C18 phases

• Compatible with highly aqueous and 100% water mobile phases

• Rapid equilibration minimizes preparation time between runs

• Suitable for hydrophobic and moderately non-polar compounds

• 10 mm ID format provides increased sample loading capacity compared to analytical columns

• Short 50 mm length minimizes run times and solvent consumption

• 5 µm particles support semi-preparative flow rates with manageable backpressure

• Typical operating range is approximately 4-8 mL/min

• Requires a semi-preparative or preparative HPLC system capable of higher flow rates and larger injection volumes

• Suitable for analytical-to-semi-preparative method scale-up

• This is a Special Order Item