Prep HPLC Column, RP C18, Wide Pore, 5um, 300A. 30mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68518‑P30‑100‑3M Cogent RP C18 Wide Pore Preparative HPLC Column is designed for production‑scale biomolecule purification and method development, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, extremely high loading capacity, and consistent chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Reliable, reproducible purification performance in high‑throughput environments
The 300 Å pore size allows large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is widely used in pilot plants and full production environments as a USP L1 alternate for large‑molecule separations.
100 mm Length – Increased Resolution for Production Development
Higher resolving power than 50 mm scouting columns
Improved separation of proteins, peptides, and complex biomolecules
Increased peak capacity for preparative purification workflows
Ideal for refining methods prior to longer columns or production runs
This configuration is selected when additional resolution is required beyond initial production‑scale screening.
30 mm ID – Maximum Production‑Scale Loading
Provides extremely high sample loading capacity
Designed for large‑scale biomolecule purification and manufacturing workflows
Supports very high‑flow preparative chromatography systems
Typical Flow Rate Range
~20–60 mL/min (system dependent)
Optimize based on instrument capability and production requirements
✅ Designed for production‑scale HPLC systems with high flow rates and large injection volumes
Flow Rate & System Considerations (30 mm ID, 5 µm, 300 Å)
Requires systems capable of:
Very high flow rates
Large injection volumes
Compatible with:
Production preparative pumps
Large‑volume injection systems
High‑capacity fraction collection systems
⚠️ Not suitable for analytical or semi‑prep HPLC systems
Why Choose Wide Pore (300 Å) C18 (5 µm, Production Scale)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore materials
High‑Resolution Production Performance
Ideal for refining and executing large‑scale purification workflows
Balances throughput with improved separation performance
Supports high‑purity biomolecule isolation
Scalable Workflow Integration
Predictable selectivity from analytical → semi‑prep → prep → production
Supports efficient process development and scale‑up
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations
High Purity Silica
Consistent chromatographic performance
Reduced variability across runs and production batches
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load production workflows
Reduced downtime and replacement cost
Typical Applications
Production‑scale biomolecule purification
Protein and peptide separations
Method development and process optimization
High‑throughput pharmaceutical manufacturing
Complex biomolecule mixtures
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires production‑scale HPLC system capability (30 mm ID)
Ideal for method refinement prior to full production runs
Optimize for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable