Catalog Number - 68518-P30-250

The 68518‑P30‑250 Cogent RP C18 Preparative HPLC Column is engineered for maximum separation performance in production‑scale purification workflows, utilizing 5 µm particles and fully double end‑capped C18 bonding to deliver reliable retention, extremely high loading capacity, and consistent chromatographic performance under demanding conditions.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this stationary phase provides:

• Complete end‑capping to minimize silanol interactions

• Improved peak symmetry for polar and basic analytes

• Reproducible results across large‑batch and continuous purification runs

This column is ideal as a USP L1 alternate and serves as a top‑tier solution for final purification and manufacturing‑scale workflows.

250 mm Length – Maximum Separation & Highest Purity

• Highest resolving power in the 30 mm RP C18 (5 µm) range

• Maximizes peak capacity for highly complex mixtures

• Ideal for closely related compounds and challenging separations

• Provides extended retention for hydrophobic analytes

• Best suited for final purification and highest‑purity isolation

This configuration is selected when shorter columns cannot achieve required resolution or purity at production scale.

30 mm ID – Maximum Production‑Scale Loading

• Provides extremely high sample loading capacity

• Designed for large‑scale purification, batch production, and manufacturing workflows

• Supports very high‑flow preparative chromatography systems

Typical Flow Rate Range

• ~20–60 mL/min (system dependent)

• Optimize based on instrument capability and production demands

✅ Designed for production‑scale HPLC systems with high flow rates and large injection volumes

Flow Rate & System Considerations (30 mm ID, 5 µm)

• Requires systems capable of:

  • Very high flow rates

  • Large injection volumes

• Compatible with:

  • Production preparative pumps

  • Large‑volume injection systems

  • High‑capacity fraction collection systems

⚠️ Not suitable for analytical or semi‑prep HPLC systems

Why Choose RP C18 (5 µm, Production Scale)

Maximum Resolution for Production Purification

• Ideal for final purification of complex mixtures at manufacturing scale

• Maximizes separation efficiency and product purity

• Supports high‑purity compound isolation workflows

Reliable Hydrophobic Retention

• Strong reversed‑phase retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids and similar analytes

• Provides greater retention than C8 phases

USP Method Compatibility

• Suitable for USP L1 methods

• Reliable for regulated production and validation environments

• Supports consistent method transfer across all HPLC scales

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for challenging analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability across production batches

Durable & Cost‑Efficient

• Long column lifetime

• Designed for high‑load production workflows

• Reduced downtime and replacement costs

Typical Applications

• Production‑scale purification

• High‑purity compound isolation

• Complex mixture separations

• Pharmaceutical manufacturing and QC

• Process development and industrial scale‑up

• USP L1 reversed‑phase methods

Important Usage Notes

• Designed for 30 mm production‑scale HPLC systems

• Requires very high‑flow capability and high sample loading capacity

• Ideal for maximum‑resolution, high‑purity workflows

• Optimize conditions for resolution vs throughput balance

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.