Catalog Number - 68522-25P

68522-25P Cogent RP PFP™ HPLC Column is a very popular choice among HPLC laboratories, delivering consistent performance and enhanced selectivity for complex separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using ultra‑high purity silica.

The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it highly effective for halogenated, aromatic, and polar analytes. It is also well suited as an alternate column for USP methods requiring L43 classification.

With a 5 µm particle size, this column offers lower backpressure compared to smaller particle columns, allowing higher flow rates and reliable, robust performance in standard analytical HPLC systems.

250 mm Length – Maximum Resolution for Complex Separations

The 250 mm length delivers the highest resolving power, making it ideal for demanding separations and complex sample matrices.

• Maximum resolution compared to shorter column formats

• Superior separation of co-eluting and closely related compounds

• Increased peak capacity for complex analytical challenges

✅ Recommended for impurity profiling, method development, and high-resolution workflows

Flow Rate & System Requirements (4.6 mm ID, 5 µm)

⚠️ Fully compatible with standard analytical HPLC systems with moderate backpressure

• Typical flow rate range: ~1.0 – 1.5 mL/min

• Lower backpressure than 3 µm columns

• Supports stable, higher flow rate operation

• Compatible with UV and standard detectors

✅ Best Practice: Filter samples using a 0.2 µm syringe filter prior to injection

Why Choose PFP (F5) Columns for Selective Separations

Enhanced Retention for Halogenated Compounds

• Strong interaction with halogenated and fluorinated analytes

• Improved selectivity vs C18 columns

Multiple Interaction Mechanisms

• π‑π interactions for aromatic compounds

• Dipole and hydrogen bonding interactions

• Greater flexibility for method development

100 Å Pore Structure

• Suitable for small to moderately sized molecules

• Broad applicability across analytical methods

Cogent RP PFP™ Column Advantages

Fully End‑Capped Surface

• Minimizes silanol activity

• Improves peak symmetry

Robust Performance (5 µm Particles)

• Lower backpressure vs smaller particle sizes

• Reliable and consistent performance

High Purity Silica

• Reproducible chromatographic performance

• Long column lifetime

Typical Applications

• Halogenated compound analysis

• Aromatic compound separations

• Pharmaceutical QA/QC workflows

• Method development and impurity profiling

• USP L43 methods

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Lower backpressure compared to smaller particle columns

• Use 0.2 µm filtration prior to injection