CUSTOM ITEM: HPLC Column, RP PFP, 5um, 4.0mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
68522-25P-4 Cogent RP PFP™ HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for complex separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using ultra‑high purity silica.
The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it highly effective for halogenated, aromatic, and polar analytes. It is also suitable as an alternate column for USP methods requiring L43 classification.
With a 5 µm particle size, this column delivers lower backpressure compared to smaller particle columns, allowing higher flow rates and robust performance while maintaining strong chromatographic efficiency.
250 mm Length – Maximum Resolution for Complex Separations
The 250 mm length provides the highest resolving power, ideal for demanding separations involving complex mixtures or closely related analytes.
Maximum resolution compared to shorter column formats
Superior separation of co-eluting compounds
Increased peak capacity for highly complex samples
✅ Recommended for impurity profiling, method development, and high-resolution analytical workflows
Flow Rate & System Requirements (4.0 mm ID, 5 µm)
⚠️ Compatible with standard analytical HPLC systems with moderate backpressure
Typical flow rate range: ~0.8 – 1.3 mL/min
Lower backpressure than 3 µm columns
Increased pressure due to extended column length
Compatible with UV and standard analytical detectors
✅ Best Practice: Filter samples using a 0.2 µm syringe filter prior to injection
Why Choose PFP (F5) Columns for Selective Separations
Enhanced Retention for Halogenated Compounds
Strong interaction with halogenated and fluorinated analytes
Improved selectivity vs C18 columns
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility in method development
100 Å Pore Structure
Suitable for small to moderately sized molecules
Broad applicability across analytical methods
Cogent RP PFP™ Column Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry
Robust Performance (5 µm Particles)
Lower backpressure vs smaller particle sizes
Reliable, consistent operation
High Purity Silica
Reproducible chromatographic performance
Long column lifetime
Typical Applications
Halogenated compound analysis
Aromatic compound separations
Pharmaceutical QA/QC workflows
Method development and impurity profiling
USP L43 methods
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Designed for standard analytical HPLC systems (4.0 mm ID)
Lower backpressure compared to smaller particle sizes
Increased pressure due to 250 mm length
Use 0.2 µm filtration prior to injection
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable