Prep HPLC Column, RP PFP, 5um, 100A. 21.2mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
68522-P21-250 Cogent RP PFP™ Prep HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering consistent performance and enhanced selectivity for demanding preparative and scale‑up separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using high purity 100 Å silica.
The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it highly effective for halogenated, aromatic, and polar analytes. These mechanisms enable superior resolution of closely related compounds, which is essential in preparative purification workflows.
With a 5 µm particle size, this column supports high flow rates typical of preparative HPLC while maintaining strong efficiency and controlled backpressure.
250 mm Length – Maximum Resolution for Preparative Purification
The 250 mm length delivers the highest resolving power in preparative formats, making it ideal for complex mixtures and difficult separations.
Maximum resolution compared to shorter column lengths
Superior separation of structurally similar and co‑eluting compounds
Highest peak capacity for complex purification challenges
✅ Recommended for impurity isolation, high‑purity targets, and large‑scale purification workflows
Flow Rate & System Requirements (21.2 mm ID, 5 µm)
⚠️ Requires preparative HPLC system capability
Typical flow rate range: ~10 – 25 mL/min
Designed for high‑flow preparative operation
Increased backpressure due to longer column length, but manageable
Compatible with fraction collectors and preparative detectors
✅ Important: A specialized preparative HPLC system is required for columns of this size
✅ Best Practice: Use appropriate sample filtration and a matched RP PFP guard column cartridge to protect the column and maintain performance
Why Choose PFP (F5) Columns for Preparative Work
Enhanced Selectivity for Complex Mixtures
Strong interaction with halogenated and fluorinated compounds
Improved selectivity compared to C18 columns
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility in purification method development
100 Å Pore Structure
Suitable for small to moderately sized molecules
Consistent performance from analytical to preparative scale
Cogent RP PFP™ Column Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry and recovery
Optimized for Preparative Flow (5 µm Particles)
Supports high flow rates
Balanced efficiency and pressure
High Purity Silica
Reliable, reproducible chromatographic performance
Consistent results during scale‑up
Typical Applications
Preparative HPLC purification
Large‑scale compound isolation
Impurity isolation and high‑purity production
Halogenated compound separations
Aromatic compound purification
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Requires preparative HPLC system capability
Designed for high‑flow operation (21.2 mm ID columns)
Increased pressure due to 250 mm length
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable