Prep HPLC Column, RP PFP, 5um, 100A. 30mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
68522-P30-250 Cogent RP PFP™ Prep HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for demanding preparative and production‑scale separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using high purity 100 Å silica.
The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it highly effective for halogenated, aromatic, and polar analytes. These interactions are critical when separating closely related compounds and achieving high purity in large‑scale workflows.
With a 5 µm particle size, this column supports very high flow rates typical of 30 mm preparative systems while maintaining strong efficiency and controlled backpressure.
250 mm Length – Maximum Resolution for Large‑Scale Purification
The 250 mm length delivers the highest resolving power available in preparative formats, ideal for complex separations and high‑purity requirements.
Maximum resolution compared to shorter column lengths
Superior separation of co‑eluting and structurally similar compounds
Highest peak capacity for complex sample matrices
✅ Recommended for impurity isolation, difficult separations, and high‑purity production workflows
Flow Rate & System Requirements (30 mm ID, 5 µm)
⚠️ Requires preparative or process‑scale HPLC system capability
Typical flow rate range: ~20 – 50 mL/min
Designed for very high‑flow preparative operation
Increased backpressure due to longer column length, but manageable
Compatible with large‑scale fraction collection systems
✅ Important: A specialized preparative or process‑scale HPLC system is required for columns of this size
✅ Best Practice: Use appropriate sample filtration and a matched RP PFP guard column cartridge to protect the column and maintain performance
Why Choose PFP (F5) Columns for Preparative Work
Enhanced Selectivity for Complex Mixtures
Strong interaction with halogenated and fluorinated compounds
Improved selectivity vs conventional C18 columns
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility for purification method development
100 Å Pore Structure
Suitable for small to moderately sized molecules
Consistent performance from analytical to large‑scale preparative systems
Cogent RP PFP™ Column Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry and compound recovery
Optimized for Preparative Flow (5 µm Particles)
Supports very high flow rates
Balanced efficiency and pressure for large-diameter columns
High Purity Silica
Reliable and reproducible chromatographic performance
Consistent purification results at scale
Typical Applications
Large‑scale preparative HPLC purification
Process-scale compound isolation
Impurity isolation and high‑purity production
Halogenated compound separations
Aromatic compound purification
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Requires preparative or process‑scale HPLC system capability
Designed for high‑flow operation (30 mm ID columns)
Increased pressure due to 250 mm column length
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable