Process HPLC Column, RP PFP, 5um, 100A. 50mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
68522-P50-250 Cogent RP PFP™ Process HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering consistent performance and enhanced selectivity for large‑scale and production‑level purification. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using high purity 100 Å silica.
The column offers multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it highly effective for halogenated, aromatic, and polar analytes. These interactions are especially valuable for resolving closely related compounds and achieving high purity in complex, process-scale separations.
With a 5 µm particle size, this column supports extremely high flow rates typical of 50 mm ID systems while maintaining strong chromatographic efficiency and controlled backpressure.
250 mm Length – Maximum Resolution at Process Scale
The 250 mm length provides the highest resolving power available in process HPLC formats, ideal for complex mixtures and high‑purity production requirements.
Maximum resolution compared to shorter column lengths
Superior separation of co‑eluting and structurally similar compounds
Highest peak capacity for complex purification challenges
✅ Recommended for impurity isolation, high‑purity production, and difficult large‑scale separations
Flow Rate & System Requirements (50 mm ID, 5 µm)
⚠️ Requires process‑scale or pilot‑scale HPLC system capability
Typical flow rate range: ~40 – 120 mL/min
Designed for ultra high‑flow operation
Increased backpressure due to column length, but manageable
Compatible with industrial fraction collection and process systems
✅ Important: A specialized process‑scale HPLC system is required for columns of this size
✅ Best Practice: Use proper sample filtration and a matched RP PFP guard column cartridge to protect the column and maintain long‑term performance
Why Choose PFP (F5) Columns for Process‑Scale Work
Enhanced Selectivity for Complex Mixtures
Strong interaction with halogenated and fluorinated compounds
Improved selectivity vs traditional C18 columns
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility in purification method development and scale‑up
100 Å Pore Structure
Suitable for small to moderately sized molecules
Consistent performance from analytical development to full production
Cogent RP PFP™ Column Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry and compound recovery
Optimized for Process Flow (5 µm Particles)
Supports extremely high flow rates
Balanced efficiency and pressure for large‑diameter systems
High Purity Silica
Reliable and reproducible chromatographic performance
Consistent purification results in production environments
Typical Applications
Process‑scale HPLC purification
Production‑scale compound isolation
Impurity isolation and high‑purity manufacturing
Halogenated compound purification
Aromatic compound separations
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Requires process‑scale HPLC system capability
Designed for ultra high‑flow operation (50 mm ID columns)
Increased pressure due to longer column length
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable