Semi Prep HPLC Column, RP PFP, 5um, 100A. 10mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
68522-SP100 Cogent RP PFP™ Semi Prep HPLC Column has been a premium value trusted by HPLC laboratories for many years, offering consistent performance and enhanced selectivity for purification and scale‑up applications. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using high purity 100 Å silica.
The column enables multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it particularly effective for halogenated, aromatic, and polar analytes. This selectivity is critical in semi‑prep workflows where accurate fractionation and compound purity are required.
With a 5 µm particle size, this column supports higher flow rates typical of semi‑preparative HPLC, balancing resolution, throughput, and system pressure.
100 mm Length – Balanced Resolution and Throughput
The 100 mm length offers an ideal balance between resolving power and run time for semi‑prep applications.
Improved resolution compared to 50 mm columns
Faster run times than longer preparative formats
Increased peak capacity for moderately complex separations
✅ Recommended for routine purification, scale‑up, and method optimization
Flow Rate & System Requirements (10 mm ID, 5 µm)
⚠️ Requires semi‑preparative or preparative HPLC system capability
Typical flow rate range: ~3.0 – 5.0 mL/min
Designed for high‑flow semi‑prep operation
Moderate backpressure relative to column size
Compatible with fraction collectors and preparative detectors
✅ Important: A specialized HPLC system capable of semi‑prep flow rates is required
✅ Best Practice: Use appropriate sample filtration or pre‑column protection to maintain column performance
Why Choose PFP (F5) Columns for Semi‑Prep Work
Enhanced Selectivity for Complex Samples
Strong interaction with halogenated and fluorinated compounds
Improved selectivity vs C18 columns
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility for method scaling
100 Å Pore Structure
Suitable for small to moderately sized molecules
Balanced performance in purification workflows
Cogent RP PFP™ Column Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry and recovery
Optimized for Semi‑Prep (5 µm Particles)
Supports higher flow rates
Maintains efficiency with manageable pressure
High Purity Silica
Reliable and reproducible performance
Consistent scaling from analytical methods
Typical Applications
Semi‑prep purification workflows
Method scaling from analytical to preparative
Halogenated compound purification
Aromatic compound isolation
Pharmaceutical development and impurity isolation
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Requires semi‑prep or preparative HPLC system
Designed for high‑flow operation (10 mm ID columns)
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable