Semi Prep HPLC Column, RP PFP, 5um, 100A. 10mm ID x 150mm Length. Cogent Brand. 1 EA..

Additional Info:

68522-SP150 Cogent RP PFP™ Semi Prep HPLC Column has been a premium value trusted by HPLC laboratories for many years, offering consistent performance and enhanced selectivity for purification and scale‑up applications. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using high purity 100 Å silica.

The column enables multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it especially effective for halogenated, aromatic, and polar analytes. This selectivity is critical in semi‑prep workflows where precise fractionation and high compound purity are required.

With a 5 µm particle size, this column supports higher flow rates typical of semi‑preparative HPLC, while delivering strong resolution and reliable performance.

150 mm Length – Enhanced Resolution for Purification

The 150 mm length provides increased resolving power compared to shorter semi‑prep columns, making it ideal for more complex separations.

  • Higher resolution than 50 mm and 100 mm formats

  • Improved separation of closely related compounds

  • Increased peak capacity for complex mixtures

✅ Recommended for purification workflows requiring higher separation performance

Flow Rate & System Requirements (10 mm ID, 5 µm)

⚠️ Requires semi‑preparative or preparative HPLC system capability

  • Typical flow rate range: ~3.0 – 5.0 mL/min

  • Designed for high‑flow semi‑prep operation

  • Moderate backpressure relative to column size

  • Compatible with fraction collectors and preparative detectors

✅ Important: A specialized HPLC system capable of semi‑prep flow rates is required

✅ Best Practice: Use appropriate sample filtration or pre‑column protection to maintain column performance

Why Choose PFP (F5) Columns for Semi‑Prep Work

Enhanced Selectivity for Complex Samples

  • Strong interaction with halogenated and fluorinated compounds

  • Improved selectivity vs C18 columns

Multiple Interaction Mechanisms

  • π‑π interactions for aromatic compounds

  • Dipole and hydrogen bonding interactions

  • Greater flexibility for method scaling and optimization

100 Å Pore Structure

  • Suitable for small to moderately sized molecules

  • Balanced performance for purification workflows

Cogent RP PFP™ Column Advantages

Fully End‑Capped Surface

  • Minimizes silanol activity

  • Improves peak symmetry and recovery

Optimized for Semi‑Prep (5 µm Particles)

  • Supports higher flow rates

  • Maintains efficiency with controlled backpressure

High Purity Silica

  • Reliable, reproducible chromatographic performance

  • Consistent results across scaling applications

Typical Applications

  • Semi‑prep purification workflows

  • Method scaling from analytical to preparative

  • Halogenated compound purification

  • Aromatic compound isolation

  • Pharmaceutical development and impurity isolation

Important Usage Notes

  • Packed and shipped in reversed‑phase solvents

  • Requires semi‑prep or preparative HPLC system

  • Designed for high‑flow operation (10 mm ID columns)

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

  • Columns are manufactured specifically to order

  • Orders cannot be canceled once placed

  • Custom columns are non‑returnable

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