Semi Prep HPLC Column, RP PFP, 5um, 100A. 10mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
68522-SP250 Cogent RP PFP™ Semi Prep HPLC Column has been a premium value trusted by HPLC laboratories for many years, offering consistent performance and enhanced selectivity for demanding purification and scale‑up applications. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using high purity 100 Å silica.
The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it highly effective for halogenated, aromatic, and polar analytes. These capabilities are especially important in semi‑prep workflows where purity, reproducibility, and resolution are critical.
With a 5 µm particle size, this column supports high flow rates typical of semi‑preparative HPLC while maintaining strong separation efficiency and manageable system backpressure.
250 mm Length – Maximum Resolution for Purification
The 250 mm length provides the highest resolving power in semi‑prep formats, ideal for complex mixtures and difficult separations.
Maximum resolution compared to shorter column lengths
Superior separation of closely related and co-eluting compounds
Highest peak capacity for complex samples
✅ Recommended for impurity isolation, complex purification, and high-resolution scale‑up workflows
Flow Rate & System Requirements (10 mm ID, 5 µm)
⚠️ Requires semi‑preparative or preparative HPLC system capability
Typical flow rate range: ~3.0 – 5.0 mL/min
Designed for high‑flow semi‑prep operation
Increased backpressure due to longer column length
Compatible with fraction collectors and preparative detectors
✅ Important: A specialized HPLC system capable of semi‑prep flow rates is required
✅ Best Practice: Use proper sample filtration and/or guard column protection to maintain column performance
Why Choose PFP (F5) Columns for Semi‑Prep Work
Enhanced Selectivity for Complex Samples
Strong interaction with halogenated and fluorinated compounds
Improved selectivity vs C18 columns
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility for method scaling and optimization
100 Å Pore Structure
Suitable for small to moderately sized molecules
Balanced performance across purification workflows
Cogent RP PFP™ Column Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry and recovery
Optimized for Semi‑Prep (5 µm Particles)
Supports higher flow rates
Maintains efficiency with controlled backpressure
High Purity Silica
Reliable and reproducible chromatographic performance
Consistent results during scale‑up
Typical Applications
Semi‑prep purification workflows
Impurity isolation and compound purification
Halogenated compound separations
Aromatic compound isolation
Pharmaceutical development and scale‑up
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Requires semi‑prep or preparative HPLC system
Designed for high‑flow operation (10 mm ID columns)
Increased backpressure due to 250 mm length
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable