Semi Prep HPLC Column, RP PFP, 5um, 100A. 10mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Info:
68522-SP50 Cogent RP PFP™ Semi Prep HPLC Column has been a premium value trusted by HPLC laboratories for many years, providing consistent performance and enhanced selectivity for purification and scale‑up applications. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using high purity 100 Å silica.
The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it especially effective for halogenated, aromatic, and polar analytes. This selectivity is particularly valuable in semi‑prep workflows where resolution and compound isolation are critical.
With a 5 µm particle size, this column supports higher flow rates typical of semi‑preparative HPLC while maintaining efficient separation performance and manageable backpressure.
50 mm Length – Fast Purification and Method Development
The 50 mm length is optimized for speed and throughput in semi‑prep applications.
Fast purification cycles and reduced run times
Lower solvent consumption compared to longer columns
Ideal for method transfer and scale‑up scouting
✅ Recommended for rapid purification, fraction collection, and method development
Flow Rate & System Requirements (10 mm ID, 5 µm)
⚠️ Requires semi‑preparative or preparative HPLC system capability
Typical flow rate range: ~3.0 – 5.0 mL/min
Designed for high‑flow semi‑prep operation
Moderate backpressure relative to column size
Compatible with fraction collectors and preparative detectors
✅ Important: A specialized HPLC system capable of semi‑prep flow rates is required
✅ Best Practice: Filter samples using an appropriate pre‑column or filtration step to maintain column performance
Why Choose PFP (F5) Columns for Semi‑Prep Work
Enhanced Selectivity for Complex Samples
Strong interaction with halogenated and fluorinated compounds
Improved selectivity compared to traditional C18 columns
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility during method scaling
100 Å Pore Structure
Suitable for small to moderately sized molecules
Balanced performance in analytical and preparative workflows
Cogent RP PFP™ Column Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry and recovery
Optimized for Semi‑Prep (5 µm Particles)
Supports higher flow rates
Maintains efficiency with manageable system pressure
High Purity Silica
Reliable and reproducible performance
Consistent scaling from analytical to semi‑prep
Typical Applications
Semi‑prep purification workflows
Method scaling from analytical to preparative
Halogenated compound purification
Aromatic compound isolation
Pharmaceutical development and impurity isolation
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Requires semi‑prep or preparative HPLC system
Designed for high‑flow operation (10 mm ID columns)
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable